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anti-ACAT1 antibody :: Rabbit ACAT1 Polyclonal Antibody

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Catalog # MBS821380
Unit / Price
  0.03 mL  /  $155 +1 FREE 8GB USB
  0.1 mL  /  $220 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

ACAT1, Polyclonal Antibody

Also Known As

Anti-ACAT1 Antibody

Product Synonym Names
ACAT; MAT; Acetyl-CoA acetyltransferase, mitochondrial; Acetoacetyl-CoA thiolase; T2
Antibody/Peptide Pairs
ACAT1 peptide (MBS822822) is used for blocking the activity of ACAT1 antibody (MBS821380)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
427
OMIM
phenotype 607809
3D Structure
ModBase 3D Structure for P24752
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Monkey
Specificity
Recognizes endogenous levels of ACAT1 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ACAT1. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-ACAT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACAT1 antibody
Rabbit polyclonal antibody to ACAT1
Applications Tested/Suitable for anti-ACAT1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-ACAT1 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Western Blot (WB) of anti-ACAT1 antibody
anti-ACAT1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ACAT1 antibody
Immunohistochemical analysis of ACAT1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-ACAT1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ACAT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
17,175 Da
NCBI Official Full Name
acetyl-CoA acetyltransferase, mitochondrial
NCBI Official Synonym Full Names
acetyl-CoA acetyltransferase 1
NCBI Official Symbol
ACAT1  [Similar Products]
NCBI Official Synonym Symbols
T2; MAT; ACAT; THIL
  [Similar Products]
NCBI Protein Information
acetyl-CoA acetyltransferase, mitochondrial; acetoacetyl-CoA thiolase; acetoacetyl Coenzyme A thiolase; acetyl-Coenzyme A acetyltransferase 1; mitochondrial acetoacetyl-CoA thiolase
UniProt Protein Name
Acetyl-CoA acetyltransferase, mitochondrial
UniProt Synonym Protein Names
Acetoacetyl-CoA thiolase; T2
UniProt Gene Name
ACAT1  [Similar Products]
UniProt Synonym Gene Names
ACAT; MAT  [Similar Products]
UniProt Entry Name
THIL_HUMAN
NCBI Summary for ACAT1
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]
UniProt Comments for ACAT1
ACAT1: Plays a major role in ketone body metabolism. Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD); also known as alpha- methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3- hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. Belongs to the thiolase family.

Protein type: Carbohydrate Metabolism - propanoate; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; EC 2.3.1.9; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Amino Acid Metabolism - lysine degradation; Acetyltransferase; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - tryptophan; Mitochondrial; Carbohydrate Metabolism - butanoate; Lipid Metabolism - fatty acid; Lipid Metabolism - synthesis and degradation of ketone bodies

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: enzyme binding; protein homodimerization activity; acetyl-CoA C-acetyltransferase activity; metal ion binding; coenzyme binding

Biological Process: response to starvation; response to hormone stimulus; ketone body catabolic process; ketone body biosynthetic process; brain development; branched chain family amino acid catabolic process; ketone body metabolic process; cellular lipid metabolic process; liver development; response to organic cyclic substance; protein homooligomerization

Disease: Alpha-methylacetoacetic Aciduria
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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