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anti-eIF2alpha antibody :: Rabbit eIF2alpha Polyclonal Antibody

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Catalog # MBS2535126
Unit / Price
  0.02 mL  /  $110 +1 FREE 8GB USB
  0.06 mL  /  $150 +1 FREE 8GB USB
  0.12 mL  /  $225 +1 FREE 8GB USB
  0.2 mL  /  $360 +1 FREE 8GB USB
Product Name

eIF2alpha, Polyclonal Antibody

Popular Item
Also Known As

eIF2alpha Polyclonal Antibody

Product Synonym Names
EIF2S1; EIF2A; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Monkey
Purity/Purification
Antigen Affinity Purified
Concentration
1 mg/ml (lot specific)
Immunogen
Synthesized peptide derived from human eIF2alpha around the non-phosphorylation site of S51.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-eIF2alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-eIF2alpha antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-eIF2alpha antibody
WB: 1:500-1:2000, IHC: 1:50-1:200, ELISA: 1:5000-1:20000

Western Blot (WB) of anti-eIF2alpha antibody
Western Blot analysis of various cells using eIF2? Polyclonal Antibody at dilution of 1:1000.
anti-eIF2alpha antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-eIF2alpha antibody
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using eIF2? Polyclonal Antibody at dilution of 1:200.
anti-eIF2alpha antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-eIF2alpha antibody
Immunofluorescence analysis of Rat kidney tissue using eIF2? Polyclonal Antibody at dilution of 1:200.
anti-eIF2alpha antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for eIF2alpha. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Calculated MW: 36
Observed MW: 38
NCBI Official Full Name
eukaryotic translation initiation factor 2 subunit 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2, subunit 1 alpha
NCBI Official Symbol
Eif2s1  [Similar Products]
NCBI Official Synonym Symbols
35kDa; Eif2a; eIF2alpha; 0910001O23Rik; 2410026C18Rik
  [Similar Products]
NCBI Protein Information
eukaryotic translation initiation factor 2 subunit 1
UniProt Protein Name
Eukaryotic translation initiation factor 2 subunit 1
UniProt Synonym Protein Names
Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha
UniProt Gene Name
Eif2s1  [Similar Products]
UniProt Synonym Gene Names
Eif2a; eIF-2-alpha; eIF-2A; eIF-2alpha  [Similar Products]
UniProt Entry Name
IF2A_MOUSE
UniProt Comments for eIF2alpha
eIF2-alpha: a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Phosphorylated by at least 4 kinases: PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Upregulated in some thyroid cancers and bronchiolo-alveolar adenocarcinomas; aberrant phosphorylation correlates with Alzheimer disease and Epstein-Barr virus infections.

Protein type: RNA-binding; Translation; Translation initiation

Cellular Component: cytoplasm; eukaryotic translation initiation factor 2 complex; eukaryotic translation initiation factor 2B complex; membrane; nucleus; ribosome; stress granule

Molecular Function: nucleic acid binding; protein binding; ribosome binding; RNA binding; translation initiation factor activity

Biological Process: cellular response to amino acid starvation; negative regulation of translation initiation in response to stress; positive regulation of defense response to virus by host; protein amino acid autophosphorylation; regulation of translation; regulation of translation initiation in response to stress; translation; translational initiation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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