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anti-mDia1 antibody :: Rabbit mDia1 Polyclonal Antibody

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Catalog # MBS9603520
Unit / Price
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  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

mDia1, Polyclonal Antibody

Popular Item
Full Product Name

mDia1 Antibody

Product Synonym Names
DIAPH1; deafness, autosomal dominant 1; DFNA1; DIA1; DIAP1; DIAP1_HUMAN; DIAPH1; Diaphanous homolog 1 (Drosophila); diaphanous homolog 1; Diaphanous related formin 1; Diaphanous-related formin-1; DRF1; FLJ25265; hDIA1; LFHL1; low frequency hearing loss 1; p140DIA; Protein diaphanous homolog 1
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O60610
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Monkey
Specificity
mDia1 antibody detects endogenous levels of mDia1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human mDia1.
Subcellular Location
Cell Membrane. Cell Projection > Ruffle Membrane. Cytoplasm > Cytoskeleton. Membrane ruffles, especially at the tip of ruffles, of motile cells.
Tissue Specificity
Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea. Expressed in platelets (PubMed:26912466).
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-mDia1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-mDia1 antibody
Function: Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape (PubMed:20937854, PubMed:21834987). Plays a role in brain development (PubMed:24781755).
Subunit Structure: Homodimer. Interacts with the GTP-bound form of RHOA. Interacts with RHOC, PFY1, MAPRE1, BAIAP2 and APC. Interacts with SCAI (By similarity). Interacts with DCAF7, via FH2 domain. Interacts with NCDN (By similarity). Interacts with RHOA, OSBPL10, OSBPL2, VIM, TUBB and DYN1 (PubMed:23325789).
Post-translational Modifications: Phosphorylation at Thr-768 is stimulated by cAMP and regulates stability, complex formation and mitochondrial movement.
Similarity: The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity). Belongs to the formin homology family. Diaphanous subfamily.
Applications Tested/Suitable for anti-mDia1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-mDia1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Western Blot (WB) of anti-mDia1 antibody
Western blot analysis of extracts of HeLa cells, using mDia1 antibody.
anti-mDia1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-mDia1 antibody
MBS9603520 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-mDia1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for mDia1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 140-150; 70 kDa
Predicted: 142 kDa
NCBI Official Full Name
protein diaphanous homolog 1 isoform 2
NCBI Official Synonym Full Names
diaphanous related formin 1
NCBI Official Symbol
DIAPH1  [Similar Products]
NCBI Official Synonym Symbols
DIA1; DRF1; DFNA1; LFHL1; SCBMS; hDIA1
  [Similar Products]
NCBI Protein Information
protein diaphanous homolog 1
UniProt Protein Name
Protein diaphanous homolog 1
UniProt Synonym Protein Names
Diaphanous-related formin-1; DRF1
UniProt Gene Name
DIAPH1  [Similar Products]
UniProt Synonym Gene Names
DIAP1; DRF1  [Similar Products]
NCBI Summary for mDia1
This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for mDia1
Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers (). Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization (). Required for cytokinesis, and transcriptional activation of the serum response factor (). DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (). Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (). Has neurite outgrowth promoting activity. Acts in a Rho-dependent manner to recruit PFY1 to the membrane (). In hear cells, it may play a role in the regulation of actin polymerization in hair cells (PubMed:20937854, PubMed:21834987, PubMed:26912466). The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex (PubMed:20937854, PubMed:21834987). It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity (PubMed:20937854, PubMed:21834987). In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (PubMed:20937854, PubMed:21834987). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape (PubMed:20937854, PubMed:21834987). Plays a role in brain development (PubMed:24781755). Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity ().
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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