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anti-MELK antibody :: MELK Polyclonal Antibody

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Catalog # MBS8212103
Unit / Price
  0.03 mL  /  $155 +1 FREE 8GB USB
  0.1 mL  /  $220 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

MELK, Polyclonal Antibody

Popular Item
Also Known As

Anti-MELK Antibody

Product Synonym Names
KIAA0175; Maternal embryonic leucine zipper kinase; hMELK; Protein kinase Eg3; pEg3 kinase; Protein kinase PK38; hPK38; Tyrosine-protein kinase MELK
Antibody/Peptide Pairs
MELK peptide (MBS8235449) is used for blocking the activity of MELK antibody (MBS8212103)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
610
OMIM
607025
3D Structure
ModBase 3D Structure for Q14680
Clonality
Polyclonal
Species Reactivity
Human, Mouse, Rat, Monkey
Specificity
Recognizes endogenous levels of MELK protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Source
Rabbit
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MELK. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-MELK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MELK antibody
Rabbit polyclonal antibody to MELK
Applications Tested/Suitable for anti-MELK antibody
Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-MELK antibody
WB (1/500 - 1/1000); IHC (1/100 - 1/200); IF (1/100 - 1/500); ICC (1/100 - 1/500)

Western Blot (WB) of anti-MELK antibody
Western blot analysis of MELK expression in MCF7 (A), NIH3T3 (B), mouse liver (C), rat liver (D) whole cell lysates.
anti-MELK antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-MELK antibody
Immunohistochemical analysis of MELK staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-MELK antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-MELK antibody
Immunofluorescent analysis of MELK staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
anti-MELK antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for MELK. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
69,116 Da
NCBI Official Full Name
maternal embryonic leucine zipper kinase isoform 2
NCBI Official Synonym Full Names
maternal embryonic leucine zipper kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
HPK38
  [Similar Products]
NCBI Protein Information
maternal embryonic leucine zipper kinase
UniProt Protein Name
Maternal embryonic leucine zipper kinase
UniProt Synonym Protein Names
Protein kinase Eg3; pEg3 kinase; Protein kinase PK38; hPK38; Tyrosine-protein kinase MELK (EC:2.7.10.2)
UniProt Gene Name
UniProt Synonym Gene Names
KIAA0175; hMELK; pEg3 kinase; hPK38  [Similar Products]
UniProt Entry Name
MELK_HUMAN
UniProt Comments for MELK
MELK: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis. Monomer. Interacts with ZNF622 and PPP1R8. Up-regulated in many cancers cells. Up-regulated upon treatment with radiation or 5-fluorouracil (5-FU) in colorectal cancer cells, suggesting that it might be associated with increased resistance of colorectal cells against radiation and 5- FU. Down-regulated upon siomycin A, a thiazole antibiotic, treatment, leading to inhibit tumor growth in vivo. Expressed in placenta, kidney, thymus, testis, ovary and intestine. Activated by autophosphorylation of the T-loop at Thr-167 and Ser-171: in contrast to other members of the SNF1 subfamily, phosphorylation at Thr-167 is not mediated by STK11/LKB1 but via autophosphorylation instead. Inhibited by calcium-binding. Kinase activity is also regulated by reducing agents: dithiothreitol (DTT) or reduced glutathione are required for kinase activity in vitro; such dependence is however not due to the presence of disulfide bonds. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Protein type: EC 2.7.10.2; RNA splicing; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, CAMK; CAMK group; CAMKL family; MELK subfamily

Chromosomal Location of Human Ortholog: 9p13.2

Cellular Component: membrane; plasma membrane; cell cortex

Molecular Function: protein serine/threonine kinase activity; protein binding; non-membrane spanning protein tyrosine kinase activity; calcium ion binding; lipid binding; ATP binding

Biological Process: cell proliferation; peptidyl-tyrosine phosphorylation; apoptosis; positive regulation of apoptosis; protein amino acid autophosphorylation; hemopoiesis; G2/M transition of mitotic cell cycle; induction of apoptosis by oxidative stress
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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