• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-Akt1 antibody :: Rabbit anti-Human, Mouse Akt Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS631310
Unit / Price
  10 Blots  /  $620 +1 FREE 8GB USB
anti-Akt1 antibody
Product Name

Akt (Akt1), Polyclonal Antibody

Also Known As

Akt, phosphorylated (Thr308) (Rac PKa, PKBa)

Product Synonym Names
Anti -Akt, phosphorylated (Thr308) (Rac PKa, PKBa)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 3R; NT_033777.2 (11924938..11930239). Location: 89B3-89B3
3D Structure
ModBase 3D Structure for Q8INB9
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Specificity
Recognizes human Akt1 when phosphorylated at Thr308. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Immunogen
Synthetic peptide corresponding to the region of human Akt1 that contains threonine 308.
Positive Control
3T3-L1 pre-adipocytes +/- PDGF treatment; HEK293 cells coexperessing RasV12 and RacL61
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of anti-Akt1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Akt1 antibody
Akt, also known as Protein Kinase B (PKB) or RAC-a, is a 60kD serine/threonine kinase that plays an important role in multiple biological responses including metabolism, cell survival and growth by phosphorylating many proteins including GSK-3b, caspase 9, BAD, and the Forkhead Transcription Factor. Akt is activated by PI3K, which in turn can interact with proteins like FAK (when FAK is phosphorylated at tyrosine 397), thereby linking activation to the cytoskeleton. Akt is phosphorylated on threonine 308 by PDK1 and on serine 473 by PDK2. Phosphorylation at threonine 308 is necessary for phosphorylation of serine 473, which is required for full activation of Akt.
Applications Tested/Suitable for anti-Akt1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-Akt1 antibody
Suitable for use in ELISA and Western Blot
Dilution: Western Blot: 1:1000
NCBI/Uniprot data below describe general gene information for Akt1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
68,485 Da[Similar Products]
NCBI Official Full Name
Akt1, isoform B
NCBI Official Symbol
NCBI Official Synonym Symbols
akt; Akt; AKT; Akt/PKB; AKT/PKB; akt1; AKT1; Akt1|PKB; CG4006; D-Akt; dakt; dAkt; dAKT; Dakt; DAkt; dAKT/dPKB; dAkt/PKB; dakt1; dAkt1; dAKT1; Dakt1; DAkt1; DAKT1; DAKT1/PKB; DmelCG4006; dPKB; Dpkb; DPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq;
  [Similar Products]
NCBI Protein Information
CG4006 gene product from transcript CG4006-RA; Akt kinase; Akt1; Akt1-PA; Akt1-PB; Akt1-PC; CG4006-PA; CG4006-PB; CG4006-PC; dAkt kinase; protein kinase B; related to PKA to PKC protein kinases; related to the A and C kinases
UniProt Protein Name
RAC serine/threonine-protein kinase
UniProt Synonym Protein Names
Akt; Protein kinase B
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
DAkt; DRAC-PK; Dakt1; PKB  [Similar Products]
UniProt Entry Name
AKT1_DROME
UniProt Comments for Akt1
Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: EC 2.7.11.1; Oncoprotein; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; AKT family

Cellular Component: cell surface; cell soma; cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase activity; ATP binding; phosphoinositide binding; protein kinase activity

Biological Process: circadian rhythm; oogenesis; positive regulation of cell size; regulation of dendrite development; chitin-based embryonic cuticle biosynthetic process; positive regulation of multicellular organism growth; protein amino acid phosphorylation; somatic muscle development; regulation of protein import into nucleus; regulation of cell shape; sequestering of lipid; myoblast fusion; negative regulation of synaptic growth at neuromuscular junction; positive regulation of axon regeneration; multicellular organism growth; regulation of multicellular organism growth; regulation of organ growth; positive regulation of organ growth; positive regulation of cell growth; epithelial cell migration, open tracheal system; dendrite regeneration; regulation of hemocyte proliferation; open tracheal system development; insulin receptor signaling pathway; response to oxidative stress; lipid metabolic process; regulation of cell size; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000