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anti-AKT1S1 antibody :: Rabbit anti-Human, Mouse AKT1S1 Polyclonal Antibody

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Catalog # MBS9125348
Unit / Price
  0.05 mL  /  $230 +1 FREE 8GB USB
  0.1 mL  /  $305 +1 FREE 8GB USB
  0.2 mL  /  $460 +1 FREE 8GB USB
Product Name

AKT1S1, Polyclonal Antibody

Also Known As

AKT1S1 Polyclonal Antibody

Product Synonym Names
Lobe; PRAS40
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
256
OMIM
610221
3D Structure
ModBase 3D Structure for Q96B36
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human AKT1S1
Calculated Molecular Weight
27kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-AKT1S1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AKT1S1 antibody
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003
Product Categories/Family for anti-AKT1S1 antibody
Applications Tested/Suitable for anti-AKT1S1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-AKT1S1 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200

Western Blot (WB) of anti-AKT1S1 antibody
Western blot analysis of extracts of various cell lines, using AKT1S1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
anti-AKT1S1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-AKT1S1 antibody
Immunohistochemistry of paraffin-embedded human liver injury using AKT1S1 Antibody at dilution of 1:100 (40x lens).
anti-AKT1S1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-AKT1S1 antibody
Immunohistochemistry of paraffin-embedded rat heart using AKT1S1 Antibody at dilution of 1:100 (40x lens).
anti-AKT1S1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-AKT1S1 antibody
Immunohistochemistry of paraffin-embedded human breast cancer using AKT1S1 Antibody at dilution of 1:100 (40x lens).
anti-AKT1S1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for AKT1S1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
256
NCBI Official Full Name
Proline-rich AKT1 substrate 1
NCBI Official Synonym Full Names
AKT1 substrate 1 (proline-rich)
NCBI Official Symbol
AKT1S1  [Similar Products]
NCBI Official Synonym Symbols
Lobe; PRAS40
  [Similar Products]
NCBI Protein Information
proline-rich AKT1 substrate 1; AKT1S1; 40 kDa proline-rich AKT substrate
UniProt Protein Name
Proline-rich AKT1 substrate 1
UniProt Synonym Protein Names
40 kDa proline-rich AKT substrate
UniProt Gene Name
AKT1S1  [Similar Products]
UniProt Entry Name
AKTS1_HUMAN
NCBI Summary for AKT1S1
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008]
UniProt Comments for AKT1S1
Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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