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anti-Prkag2 antibody :: rabbit anti-Human, Mouse AMP Activated Protein Kinase gamma1 Polyclonal Antibody

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Catalog # MBS614598
Unit / Price
  0.1 mL  /  $540 +1 FREE 8GB USB
anti-Prkag2 antibody
Product Name

AMP Activated Protein Kinase gamma1 (Prkag2), Polyclonal Antibody

Also Known As

AMP Activated Protein Kinase gamma1 (AMPK g1)

Product Synonym Names
Anti -AMP Activated Protein Kinase gamma1 (AMPK g1)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 4; NC_005103.2 (5620992..5667438). Location: 4q11
3D Structure
ModBase 3D Structure for Q6V7V4
Clonality
Polyclonal
Host
rabbit
Species Reactivity
Human, Mouse
Specificity
Detects endogenous levels of AMPKg1 protein. Species Crossreactivity: Human Mouse
Purity/Purification
Affinity Purified
Protein A and peptde affinity chromatography.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol.
Immunogen
Synthetic peptide (KLH-coupled) corresponding to residues near the amino-terminus of human AMPKg1.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-Prkag2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Prkag2 antibody
AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and
Applications Tested/Suitable for anti-Prkag2 antibody
Western Blot (WB)
Application Notes for anti-Prkag2 antibody
Suitable for use in Western Blotting.
Dilution: Western Blotting: 1:1000
NCBI/Uniprot data below describe general gene information for Prkag2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
37,311 Da[Similar Products]
NCBI Official Full Name
5'-AMP-activated protein kinase subunit gamma-2
NCBI Official Synonym Full Names
protein kinase, AMP-activated, gamma 2 non-catalytic subunit
NCBI Official Symbol
Prkag2  [Similar Products]
NCBI Official Synonym Symbols
Prkag2
  [Similar Products]
NCBI Protein Information
5'-AMP-activated protein kinase subunit gamma-2; AMPK gamma 2; AMP-activated protein kinase gamma2 subunit; adenosine monophosphate-activated protein kinase gamma 2-subunit
UniProt Protein Name
AMP-activated protein kinase gamma 2 non-catalytic subunit
UniProt Gene Name
Prkag2  [Similar Products]
UniProt Entry Name
Q6V7V4_RAT
NCBI Summary for Prkag2
predicted to act as a non-catalytic subunit of an AMP-activated protein kinase [RGD]
UniProt Comments for Prkag2
AMPKG2: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non- catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2. Isoform B is ubiquitously expressed except in liver and thymus. The highest level is detected in heart with abundant expression in placenta and testis. Belongs to the 5'-AMP-activated protein kinase gamma subunit family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, regulatory subunit; Autophagy

Cellular Component: extracellular space; AMP-activated protein kinase complex

Molecular Function: AMP-activated protein kinase activity; cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; phosphorylase kinase regulator activity; ADP binding; protein kinase binding; protein kinase activator activity; ATP binding; AMP binding

Biological Process: glycogen metabolic process; regulation of catalytic activity; regulation of glycolysis; positive regulation of protein kinase activity; regulation of fatty acid metabolic process; negative regulation of protein kinase activity; protein amino acid phosphorylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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