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anti-APOB antibody :: Rabbit anti-Human, Mouse Apolipoprotein B48/100 Polyclonal Antibody

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Catalog # MBS611063
Unit / Price
  1 mL  /  $515 +1 FREE 8GB USB
anti-APOB antibody
Product Name

Apolipoprotein B48/100 (APOB), Polyclonal Antibody

Also Known As

Apolipoprotein B48/100 (Apo B48/100)

Product Synonym Names
Anti -Apolipoprotein B48/100 (Apo B48/100)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 2; NC_000002.11 (21224301..21266945, complement). Location: 2p24-p23
OMIM
107730
3D Structure
ModBase 3D Structure for P04114
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Specificity
Recognizes mouse Apo B48 (241kD) and B100 (513kD) as these proteins are highly conserved. Species Crossreactivity: human
Purity/Purification
Serum
Heat inactivated serum
Form/Format
Supplied as a liquid, 0.05% sodium azide.
Immunogen
Mouse low density lipoprotein (LDL)
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of anti-APOB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-APOB antibody
Apolipoprotein B plays an essential role in lipid transport and metabolism. Apo B may regulate cholesterol synthesis through its interaction with specific cell membrane receptors and by inhibition of HMG CoA reductase. This enzyme has been identified as the rate controlling enzyme in cholesterol biosynthesis. Apo B may be important in the genesis of atherosclerosis. Apo B is the major protein moiety of all lipoproteins other than high density lipoprotein (HDL) in plasma. Increased levels of apo B100 are associated with an increased risk of coronary artery disease. Apo B exists in human plasma as two isoforms: apo B48 and apo B100. Apo B100 is the major physiological ligand for the LDL receptor. It contains 4536aa residues. Apo B100 is the full length protein. Its gene has been mapped on the short arm of chromosome 2, with an ~length of 43kb and 29 exons. Apo B100 is synthesised in the liver. It is required for the assembly of very low density lipoproteins (VLDL). It does not interchange between lipoprotein particles, as do the other lipoproteins. It is found in IDL and LDL particles after the removal of the apolipoproteins A, E and C. Apo B48 is present in chylomicrons and chylomicron remnants. It plays an essential role in the intestinal absorption of dietary fats. Apo B48 is synthesized in the small intestine. It comprises the N-terminal 48% of apo B100. Apo B48 is the degraded protein. Apo B48 lacks the LDL receptor-binding domain of Apo B100. It is produced due to post-transscriptional Apo B100 mRNA editing at codon 2153, which creates a stop codon in the intestine instead of a glutamine in the liver.
Product Categories/Family for anti-APOB antibody
Applications Tested/Suitable for anti-APOB antibody
Western Blot (WB)
Application Notes for anti-APOB antibody
Suitable for use in Radial Immunodiffusion (RID) and Western Blot.
Titer: RID End-Point Titer: 1:15
NCBI/Uniprot data below describe general gene information for APOB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
515,605 Da[Similar Products]
NCBI Official Full Name
apolipoprotein B-100
NCBI Official Synonym Full Names
apolipoprotein B (including Ag(x) antigen)
NCBI Official Symbol
NCBI Official Synonym Symbols
FLDB; LDLCQ4
  [Similar Products]
NCBI Protein Information
apolipoprotein B-100; apoB-48; apoB-100; apo B-100; mutant Apo B 100; OTTHUMP00000115994; apolipoprotein B48
UniProt Protein Name
Apolipoprotein B-100
Protein Family
UniProt Gene Name
UniProt Entry Name
APOB_HUMAN
NCBI Summary for APOB
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq]
UniProt Comments for APOB
APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Protein type: Secreted, signal peptide; Carrier; Secreted

Chromosomal Location of Human Ortholog: 2p24-p23

Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum membrane; intracellular membrane-bound organelle; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol; actin cytoskeleton; chylomicron; cell soma; cytoplasm; plasma membrane; endosome membrane

Molecular Function: heparin binding; protein binding; low-density lipoprotein receptor binding; cholesterol transporter activity; phospholipid binding

Biological Process: lipoprotein catabolic process; phototransduction, visible light; response to lipopolysaccharide; response to carbohydrate stimulus; post-embryonic development; response to selenium ion; triacylglycerol catabolic process; retinoid metabolic process; transmembrane transport; cholesterol metabolic process; nervous system development; receptor-mediated endocytosis; cholesterol transport; in utero embryonic development; regulation of cholesterol biosynthetic process; response to virus; cholesterol efflux; lipoprotein metabolic process; sperm motility; cholesterol homeostasis; fertilization; lipoprotein transport; lipoprotein biosynthetic process; artery morphogenesis; spermatogenesis; triacylglycerol mobilization; blood coagulation; leukocyte migration

Disease: Hypercholesterolemia, Autosomal Dominant, Type B; Hypobetalipoproteinemia, Familial, 1
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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