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anti-APPL1 antibody :: Rabbit anti-Human, Mouse APPL1 Polyclonal Antibody

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Catalog # MBS9605439
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

APPL1, Polyclonal Antibody

Popular Item
Also Known As

APPL1 Antibody

Product Synonym Names
Adapter protein containing PH domain; Adaptor Protein Containing pH Domain; Adaptor protein containing pH domain PTB domain and leucine zipper motif 1; Adaptor protein containing pH domain PTB domain and leucine zipper motif; AKT2 INTERACTOR; APPL 1; Appl1; DCC interacting protein 13 alpha; DCC-interacting protein 13-alpha; DIP 13 alpha; DIP13 alpha; Dip13-alpha; DIP13alpha; DP13A_HUMAN; KIAA1428; PTB domain and leucine zipper motif 1; PTB Domain and Leucine Zipper Motif; Signaling adaptor protein DIP13alpha
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
709
3D Structure
ModBase 3D Structure for Q9UKG1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Specificity
APPL1 antibody detects endogenous levels of total APPL1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide
Subcellular Location
Early Endosome Membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
Tissue Specificity
High levels in heart, ovary, pancreas and skeletal muscle.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-APPL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-APPL1 antibody
Function: Adapter protein that interacts with proteins involved in different cellular signaling pathways. Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction. Involved in the regulation of the insulin receptor signaling pathway.
Subunit Structure: Binds RAB5A/Rab5 through an N-terminal domain. This interaction is essential for its recruitment to endosomal membranes as well as its role in cell proliferation. Binds DCC and the catalytic domain of the inactive form of AKT2 through its PID domain. Binds PIK3CA and subunits of the NuRD/MeCP1 complex. Interacts with OCRL and INPP5B. Interacts with NTRK2 (By similarity).
Post-translational Modifications: Phosphorylation at Ser-410 by PKA severely impairs binding to OCRL.
Similarity: Overexpression of an N-terminal domain (residues 1-319) or a C-terminal region (residues 273-709) has a proapoptotic effect.The F&H motif, an approximately 12-13 amino-acid sequence centered around Phe and His residues, is essential for binding to OCRL and INPP5B.
Applications Tested/Suitable for anti-APPL1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-APPL1 antibody
WB: 1:500-1:1000
IF/ICC: 1:100-1:500

Western Blot (WB) of anti-APPL1 antibody
Western blot analysis of extracts from A549 cells, using APPL1 antibody.The lane on the left is treated with the antigen-specific peptide.
anti-APPL1 antibody Western Blot (WB) (WB) image
Immunofluorescene (IF) of anti-APPL1 antibody
MBS9605439 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
anti-APPL1 antibody Immunofluorescene (IF) image
Western Blot (WB) of anti-APPL1 antibody
Western blot analysis of extracts from A549 cells, using APPL1 antibody.The lane on the left is treated with the antigen-specific peptide.
anti-APPL1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for APPL1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 80 kD
Predicted: 80 kDa
NCBI Official Full Name
DCC-interacting protein 13-alpha
NCBI Official Synonym Full Names
adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1
NCBI Official Symbol
APPL1  [Similar Products]
NCBI Official Synonym Symbols
APPL; MODY14; DIP13alpha
  [Similar Products]
NCBI Protein Information
DCC-interacting protein 13-alpha
UniProt Protein Name
DCC-interacting protein 13-alpha
UniProt Synonym Protein Names
Adapter protein containing PH domain, PTB domain and leucine zipper motif 1
Protein Family
UniProt Gene Name
APPL1  [Similar Products]
UniProt Synonym Gene Names
; Dip13-alpha  [Similar Products]
NCBI Summary for APPL1
The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008]
UniProt Comments for APPL1
Adapter protein that interacts with proteins involved in different cellular signaling pathways. Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction. Involved in the regulation of the insulin receptor signaling pathway.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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