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anti-ASAH2 antibody :: Rabbit anti-Human, mouse ASAH2 Polyclonal Antibody

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Catalog # MBS9210331
Unit / Price
  0.08 mL  /  $165 +1 FREE 8GB USB
  0.4 mL  /  $370 +1 FREE 8GB USB
Western Blot (WB)
Product Name

ASAH2, Polyclonal Antibody

Popular Item
Full Product Name

ASAH2 Antibody (C-term)

Product Synonym Names
Neutral ceramidase; N-CDase; NCDase; Acylsphingosine deacylase 2; BCDase; LCDase; hCD; N-acylsphingosine amidohydrolase 2; Non-lysosomal ceramidase; Neutral ceramidase soluble form; ASAH2; HNAC1
Antibody/Peptide Pairs
ASAH2 peptide (MBS9222767) is used for blocking the activity of ASAH2 antibody (MBS9210331)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
734-762
OMIM
611202
3D Structure
ModBase 3D Structure for Q9NR71
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human, mouse
Specificity
This ASAH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 734-762 amino acids from the C-terminal region of human ASAH2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (lot specific)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Function
Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract.
Cellular Location
Cell membrane; Single-pass type II membrane protein. Note=The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial. However, they used a shorter form in its N- terminus, which may explain this localization which probably does not exist in vivo
Tissue Location
Primarily expressed in the intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). According to PubMed:17334805, ubiquitous expression attributed to ASAH2 may be actually that of the paralog ASAH2B.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-ASAH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ASAH2 antibody
Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze
hydrolysis of the N-acyl linkage of ceramide, a second messenger in
a variety of cellular events, to produce sphingosine. Sphingosine
exerts both mitogenic and apoptosis-inducing activities, and its
phosphorylated form functions as an intra- and intercellular second
messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed
11328816]).
Applications Tested/Suitable for anti-ASAH2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-ASAH2 antibody
WB~~1:1000

Western Blot (WB) of anti-ASAH2 antibody
ASAH2 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the ASAH2 antibody detected the ASAH2 protein (arrow).
anti-ASAH2 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ASAH2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
85516
NCBI Official Full Name
neutral ceramidase isoform b
NCBI Official Synonym Full Names
N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
NCBI Official Symbol
ASAH2  [Similar Products]
NCBI Official Synonym Symbols
HNAC1; BCDase; LCDase; NCDase; N-CDase
  [Similar Products]
NCBI Protein Information
neutral ceramidase
UniProt Protein Name
Neutral ceramidase
UniProt Synonym Protein Names
Acylsphingosine deacylase 2; BCDase; LCDase; hCD
Protein Family
UniProt Gene Name
ASAH2  [Similar Products]
UniProt Synonym Gene Names
HNAC1; N-CDase; NCDase; hCD  [Similar Products]
UniProt Entry Name
ASAH2_HUMAN
NCBI Summary for ASAH2
Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008]
UniProt Comments for ASAH2
ASAH2: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. Belongs to the neutral ceramidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - sphingolipid; Membrane protein, integral; EC 3.5.1.23; Hydrolase

Chromosomal Location of Human Ortholog: 10q11.21

Cellular Component: mitochondrion; plasma membrane; integral to membrane

Molecular Function: ceramidase activity

Biological Process: sphingolipid metabolic process; apoptosis; glycosphingolipid metabolic process; signal transduction; ceramide metabolic process
Product References and Citations for anti-ASAH2 antibody
Uchida, Y., et al. J. Invest. Dermatol. 130(10):2472-2480(2010)
Hong, K.K., et al. J. Korean Med. Sci. 22(5):862-867(2007)
Ohlsson, L., et al. Biochimie 89(8):950-960(2007)
Avramopoulos, D., et al. Neurogenetics 8(2):111-120(2007)
Galadari, S., et al. Biochem. J. 393 (PT 3), 687-695 (2006) :

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Organs/Tissues associated with anti-ASAH2 antibody
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