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anti-ASAP2 antibody :: Rabbit anti-Human, Mouse ASAP2 Polyclonal Antibody

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Catalog # MBS9609758
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Western Blot (WB)
Product Name

ASAP2, Polyclonal Antibody

Popular Item
Also Known As

ASAP2 Antibody

Product Synonym Names
AMAP 2; AMAP2; ANK repeat and PH domain-containing protein 2; Arf-GAP with SH3 domain; ArfGAP with SH3 domain, ankyrin repeat and PH domain 2; ASAP2; ASAP2_HUMAN; centaurin, beta 3; CENTB3; DDEF 2; DDEF2; Development and differentiation enhancing factor 2; Development and differentiation-enhancing factor 2; FLJ42910; KIAA0400; PAG 3; PAG3; Pap alpha; PAP; Paxillin associated protein with ARFGAP activity 3; Paxillin-associated protein with ARF GAP activity 3; Pyk2 C terminus associated protein; Pyk2 C-terminus-associated protein; SHAG 1; SHAG1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
961
3D Structure
ModBase 3D Structure for O43150
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Specificity
ASAP2 antibody detects endogenous levels of total ASAP2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human ASAP2
Subcellular Location
Cytoplasm. Golgi Apparatus > Golgi Stack Membrane. Cell Membrane. Colocalizes with F-actin and ARF6 in phagocytic cups.
Tissue Specificity
Detected in heart, brain, placenta, kidney, monocytes and pancreas.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-ASAP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ASAP2 antibody
Function: Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration.
Subunit Structure: Binds PXN, ARF1, ARF5, ARF6, PTK2B and SRC.
Post-translational Modifications: Phosphorylated on tyrosine residues by SRC and PTK2B.
Similarity: The conserved Arg-464 in the Arf-GAP domain probably becomes part of the active site of bound small GTPases and is necessary for GTP hydrolysis.
Applications Tested/Suitable for anti-ASAP2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-ASAP2 antibody
WB: 1:1000-3000

Western Blot (WB) of anti-ASAP2 antibody
Western blot analysis of ASAP2 using LOVO whole cell lysates
anti-ASAP2 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ASAP2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 112 kDa
Predicted: 112 kDa
NCBI Official Full Name
arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform b
NCBI Official Synonym Full Names
ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
NCBI Official Symbol
ASAP2  [Similar Products]
NCBI Official Synonym Symbols
PAP; PAG3; AMAP2; DDEF2; SHAG1; CENTB3; Pap-alpha
  [Similar Products]
NCBI Protein Information
arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2
UniProt Protein Name
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2
UniProt Synonym Protein Names
Development and differentiation-enhancing factor 2; Paxillin-associated protein with ARF GAP activity 3; PAG3; Pyk2 C-terminus-associated protein; PAP
UniProt Gene Name
ASAP2  [Similar Products]
UniProt Synonym Gene Names
DDEF2; KIAA0400; PAG3; PAP  [Similar Products]
NCBI Summary for ASAP2
This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
UniProt Comments for ASAP2
Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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