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anti-ATG4A antibody :: Rabbit anti-Human, mouse ATG4A Polyclonal Antibody

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Catalog # MBS7002569
Unit / Price
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Western Blot (WB)
Product Name

ATG4A, Polyclonal Antibody

Popular Item
Also Known As

ATG4A Antibody

Product Synonym Names
AUT-like 2 cysteine endopeptidase; Autophagin-2; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; hAPG4A; ATG4A; APG4A; AUTL2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
398
OMIM
300663
3D Structure
ModBase 3D Structure for Q8WYN0
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, mouse
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Immunogen
Recombinant human Cysteine protease ATG4A protein(1-214AA)
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-131433 / sc-131435 / sc-131432
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-ATG4A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ATG4A antibody
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Applications Tested/Suitable for anti-ATG4A antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-ATG4A antibody
Recommended dilution: WB:1:500-2000

Western Blot (WB) of anti-ATG4A antibody
Western blot
All lanes: ATG4A antibody at 2ug/ml+mouse brain tissue
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 46,39,37,43 kDa
Observed band size: 45 kDa

anti-ATG4A antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ATG4A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42,453 Da
NCBI Official Full Name
cysteine protease ATG4A isoform a
NCBI Official Synonym Full Names
autophagy related 4A cysteine peptidase
NCBI Official Symbol
ATG4A  [Similar Products]
NCBI Official Synonym Symbols
APG4A; AUTL2
  [Similar Products]
NCBI Protein Information
cysteine protease ATG4A
UniProt Protein Name
Cysteine protease ATG4A
UniProt Synonym Protein Names
AUT-like 2 cysteine endopeptidase; Autophagin-2; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; hAPG4A
Protein Family
UniProt Gene Name
ATG4A  [Similar Products]
UniProt Synonym Gene Names
APG4A; AUTL2; hAPG4A  [Similar Products]
UniProt Entry Name
ATG4A_HUMAN
NCBI Summary for ATG4A
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Comments for ATG4A
ATG4A: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.22.-; Microtubule-binding; Autophagy

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: cytoplasm; cytosol

Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; protein binding

Biological Process: autophagic vacuole formation; C-terminal protein lipidation; mitochondrion degradation; protein delipidation; protein processing; protein targeting to membrane; proteolysis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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