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anti-ATG4C antibody :: Rabbit anti-Human, Mouse ATG4C Polyclonal Antibody

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Catalog # MBS1493458
Unit / Price
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  0.05 mL  /  $120 +1 FREE 8GB USB
  0.1 mL  /  $160 +1 FREE 8GB USB
Product Name

ATG4C, Polyclonal Antibody

Popular Item
Full Product Name

Rabbit anti-human ATG4C polyclonal Antibody

Product Synonym Names
AUT-like 3 cysteine endopeptidase; Autophagin-3; Autophagy-related cysteine endopeptidase 3; Autophagy-related protein 4 homolog C; ATG4C; APG4C; AUTL1; AUTL3
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
611339
3D Structure
ModBase 3D Structure for Q96DT6
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Antigen Affinity Purified
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human Cysteine protease ATG4C protein (259-458aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-163708 / sc-163707
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-ATG4C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ATG4C antibody
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation (By similarity). Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Applications Tested/Suitable for anti-ATG4C antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ATG4C antibody
Recommended dilution: WB:1:200-1000
IHC:1:20-1:200

Western Blot (WB) of anti-ATG4C antibody
Western blot
All lanes: ATG4C antibody at 9ug/ml
Lane 1:mouse lung tissue
Lane 2:HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 52kDa
Observed band size: 52kDa

anti-ATG4C antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ATG4C antibody
Immunohistochemistry of paraffin-embedded human breast cancer using MBS1493458 at dilution 1:100
anti-ATG4C antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ATG4C. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
52,497 Da
NCBI Official Full Name
cysteine protease ATG4C
NCBI Official Synonym Full Names
autophagy related 4C, cysteine peptidase
NCBI Official Symbol
ATG4C  [Similar Products]
NCBI Official Synonym Symbols
APG4C; AUTL1; AUTL3; APG4-C
  [Similar Products]
NCBI Protein Information
cysteine protease ATG4C
UniProt Protein Name
Cysteine protease ATG4C
UniProt Synonym Protein Names
AUT-like 3 cysteine endopeptidase; Autophagin-3; Autophagy-related cysteine endopeptidase 3; Autophagy-related protein 4 homolog C
Protein Family
UniProt Gene Name
ATG4C  [Similar Products]
UniProt Synonym Gene Names
APG4C; AUTL1; AUTL3  [Similar Products]
UniProt Entry Name
ATG4C_HUMAN
NCBI Summary for ATG4C
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Comments for ATG4C
ATG4C: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Highly expressed in skeletal muscle, heart, liver and testis. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family.

Protein type: Protease; EC 3.4.22.-; Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: cytoplasm; cytosol; extracellular region

Molecular Function: cysteine-type endopeptidase activity; peptidase activity

Biological Process: autophagic vacuole formation; autophagy; C-terminal protein lipidation; mitochondrion degradation; protein delipidation; protein processing; protein targeting to membrane; proteolysis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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