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anti-SH3GLB1 antibody :: Rabbit anti-Human, Mouse Bif Polyclonal Antibody

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Catalog # MBS150193
Unit / Price
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  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

Bif (SH3GLB1), Polyclonal Antibody

Full Product Name

Bif Antibody

Product Synonym Names
Bif; Bif-1; CGI-61; PPP1R70; dJ612B15.2; KIAA0491; Endophilin-B1; Bax-interacting factor 1; Bif-1; SH3-domain GRB2-like endophilin B1
Antibody/Peptide Pairs
Bif peptide (MBS152090) is used for blocking the activity of Bif antibody (MBS150193)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
609287
3D Structure
ModBase 3D Structure for Q9Y371
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Bif Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
BIF antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human BIF.
Buffer
Bif Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
Bif antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-SH3GLB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SH3GLB1 antibody
Bif Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family such as Bax appear to control the activation of these enzymes. Bax activity was found to be regulated by its association with Bax-interacting factor 1 (BIF), a member of the endophilin B family that is associated with intracellular membranes. Following this interaction, Bax undergoes a conformational change and translocates to mitochondrial membranes. The Bax/BIF interaction appears to be enhanced by apoptotic stimuli, suggesting that BIF acts as the trigger to activate Bax, and as suppression of BIF promoted HeLa cell colony formation in soft agar, it may have a role in the suppression of cancer progression. At least two isoforms of BIF are known to exist.
Applications Tested/Suitable for anti-SH3GLB1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-SH3GLB1 antibody
BIF antibody can be used for the detection of BIF by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL.

Western Blot (WB) of anti-SH3GLB1 antibody
Western blot analysis of BIF in HeLa cell lysate with BIF antibody at (A) 1 and (B) 2 μg/mL.
anti-SH3GLB1 antibody Western Blot (WB) (WB) image
Immunocytochemistry (ICC) of anti-SH3GLB1 antibody
Immunocytochemistry of BIF in HeLa with BIF antibody at 10 μg/mL.
anti-SH3GLB1 antibody Immunocytochemistry (ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for SH3GLB1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,318 Da
NCBI Official Full Name
Bax-interacting factor 1
NCBI Official Synonym Full Names
SH3-domain GRB2-like endophilin B1
NCBI Official Symbol
SH3GLB1  [Similar Products]
NCBI Official Synonym Symbols
Bif-1; CGI-61; PPP1R70; dJ612B15.2
  [Similar Products]
NCBI Protein Information
endophilin-B1; Bax-interacting factor 1; SH3-containing protein SH3GLB1; SH3 domain-containing GRB2-like protein B1; protein phosphatase 1, regulatory subunit 70
UniProt Protein Name
Endophilin-B1
UniProt Synonym Protein Names
Bax-interacting factor 1; Bif-1; SH3 domain-containing GRB2-like protein B1
Protein Family
UniProt Gene Name
SH3GLB1  [Similar Products]
UniProt Synonym Gene Names
KIAA0491; Bif-1  [Similar Products]
UniProt Entry Name
SHLB1_HUMAN
NCBI Summary for SH3GLB1
This gene encodes a SRC homology 3 domain-containing protein. The encoded protein interacts with the proapoptotic member of the Bcl-2 family, Bcl-2-associated X protein (Bax) and may be involved in regulating apoptotic signaling pathways. This protein may also be involved in maintaining mitochondrial morphology. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
UniProt Comments for SH3GLB1
SH3GLB1: May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion. Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1. Homodimer, and heterodimer with SH3GLB2. Binds BAX. Induction of apoptosis augments BAX binding. Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes. Belongs to the endophilin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Mitochondrial; EC 2.3.1.-; Transferase; Autophagy

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: Golgi membrane; mitochondrial outer membrane; cytoplasm

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; lipid binding

Biological Process: positive regulation of protein oligomerization; apoptosis; protein oligomerization
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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