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anti-CDH15 antibody :: Sheep anti-Human, Mouse Cadherin-15 Polyclonal Antibody

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Catalog # MBS624331
Unit / Price
  0.1 mg  /  $765 +1 FREE 8GB USB
anti-CDH15 antibody
Product Name

Cadherin-15 (CDH15), Polyclonal Antibody

Also Known As

Cadherin-15 (Cadherin, M, Muscle Cadherin, M-cadherin, Cadherin-14, CDH15, CDH14, CDH3)

Product Synonym Names
Anti -Cadherin-15 (Cadherin, M, Muscle Cadherin, M-cadherin, Cadherin-14, CDH15, CDH14, CDH3)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 16; NC_000016.9 (89238163..89261900). Location: 16q24.3
OMIM
114019
3D Structure
ModBase 3D Structure for P55291
Clonality
Polyclonal
Isotype
IgG
Host
Sheep
Species Reactivity
Human, Mouse
Specificity
Recognizes human Cadherin-15 at ~130kD. Species Crossreactivity: mouse
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Immunogen
Recombinant corresponding to Val22-Ala606 from human Cadherin-15, expressed in NSO cells (P55291).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Other Notes
Small volumes of anti-CDH15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CDH15 antibody
Cadherin-15 is a 124kD type I transmembrane glycoprotein of the Cadherin superfamily of calcium-dependent homotypic adhesion molecules. Like other classical Cadherins, the 814aa human Cadherin-15 contains a signal sequence (21aa), a propeptide (29aa), an extracellular domain with five Cadherin domain repeats (ECD, 556aa), a transmembrane segment (20aa) and a cytoplasmic domain (188aa). The Cadherin repeats are responsible for cell-cell adhesion by homophilic binding on opposing cells. Intracellularly, Cadherin-15 binds beta-catenin or plakoglobin (gamma-catenin), which in turn bind alpha-catenin. Cadherin-15 also binds p120 catenin. Cadherin-15 is present during early stages of skeletal muscle development and is thought to align myoblasts for fusion. It is also present in muscle satellite cells and participates in muscle regeneration. It is expressed in the granule cell layer of the cerebellar glomerulus. Deletion of mouse Cadherin-15 has little effect in vivo, most likely due to compensation by N-Cadherin. However, Cadherin-15 upregulation and adhesion between myoblasts during induction of differentiation in vitro is required for their fusion. Human Cadherin-15 ECD shows 88% aa identity with mouse, rat, or bovine and 85% aa identity with canine Cadherin-15 ECD. Cadherin-15 is an outlier among classical Cadherins, with 40% aa identity or less in the ECD.
Product Categories/Family for anti-CDH15 antibody
Applications Tested/Suitable for anti-CDH15 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-CDH15 antibody
Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunocytochemistry.
Dilution: Flow Cytometry: 2.5ug labels 10e6 cells from the C2C12 mouse myoblast cell line.
Western Blot: 1ug/ml detects Caderin-15 in HeLa cells.
Immunocytochemistry: 5-15ug/ml detects Cadherin-15 in immersion fixed C2C12 mouse cells.
NCBI/Uniprot data below describe general gene information for CDH15. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
88,916 Da[Similar Products]
NCBI Official Full Name
cadherin-15 preproprotein
NCBI Official Synonym Full Names
cadherin 15, type 1, M-cadherin (myotubule)
NCBI Official Symbol
CDH15  [Similar Products]
NCBI Official Synonym Symbols
CDH3; CDHM; MCAD; MRD3; CDH14
  [Similar Products]
NCBI Protein Information
cadherin-15; cadherin-3; cadherin-14; muscle-cadherin; OTTHUMP00000175347
UniProt Protein Name
Cadherin-15
UniProt Synonym Protein Names
Cadherin-14; Muscle cadherin
Protein Family
UniProt Gene Name
CDH15  [Similar Products]
UniProt Synonym Gene Names
CDH14; CDH3  [Similar Products]
UniProt Entry Name
CAD15_HUMAN
NCBI Summary for CDH15
This gene is a member of the cadherin superfamily of genes, encoding calcium-dependent intercellular adhesion glycoproteins. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells. The protein is thought to be essential for the control of morphogenetic processes, specifically myogenesis, and may provide a trigger for terminal muscle cell differentiation. [provided by RefSeq]
UniProt Comments for CDH15
CDH15: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation. A chromosomal aberration involving CDH15 and KIRREL3 is found in a patient with severe mental retardation and dysmorphic facial features. Translocation t(11;16)(q24.2;q24). Defects in CDH15 are the cause of mental retardation autosomal dominant type 3 (MRD3). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: integral to membrane; plasma membrane; caveola; neuromuscular junction

Molecular Function: calcium ion binding

Biological Process: intercellular junction assembly and maintenance; muscle cell differentiation; positive regulation of muscle cell differentiation; homophilic cell adhesion; cell adhesion

Disease: Mental Retardation, Autosomal Dominant 3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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