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anti-CARD11 antibody :: Rabbit anti-Human, Mouse CARMA1 Polyclonal Antibody

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Catalog # MBS151587
Unit / Price
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  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

CARMA1 (CARD11), Polyclonal Antibody

Full Product Name

CARMA1 Antibody

Product Synonym Names
CARMA1; PPBL; BENTA; BIMP3; IMD11; CARMA1; Caspase recruitment domain-containing protein 11; CARD-containing MAGUK protein 1; Carma 1; caspase recruitment domain family, member 11
Antibody/Peptide Pairs
CARMA1 peptide (MBS153160) is used for blocking the activity of CARMA1 antibody (MBS151587)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
gene 615206
3D Structure
ModBase 3D Structure for Q9BXL7
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
CARMA1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
CARMA1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARMA1.
Buffer
CARMA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
CARMA1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-CARD11 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CARD11 antibody
CARMA1 Antibody: CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. Both CARMA1 and CARMA3 associate with NEMO, the regulatory subunit of the IκK complex, thereby regulating activation of the NF-κB transcription factor. Also, gene inactivation studies showed a complete block in T and B cell immunity as well as an impaired response to LPS, indicating that CARMA1 is a critical regulator in both the adaptive and innate immune systems.
Applications Tested/Suitable for anti-CARD11 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-CARD11 antibody
CARMA1 antibody can be used for detection of CARMA1 by Western blot at 0.5 to 2 mug/mL. A band at approximately 125 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-CARD11 antibody
Western blot analysis of CARMA1 expression in mouse thymus cell lysate with CARMA1 antibody at 0.5 (lane A), 1 (lane B), and 2 μg /ml (lane C), respectively.
anti-CARD11 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-CARD11 antibody
Immunohistochemistry of CARMA1 in mouse thymus with CARMA1 antibody at 10 μg/mL.
anti-CARD11 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-CARD11 antibody
Immunofluorescence of CARMA1 in Mouse Spleen cells with CARMA1 antibody at 20 μg/mL.
anti-CARD11 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for CARD11. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
133,284 Da
NCBI Official Full Name
Caspase recruitment domain-containing protein 11
NCBI Official Synonym Full Names
caspase recruitment domain family, member 11
NCBI Official Symbol
CARD11  [Similar Products]
NCBI Official Synonym Symbols
PPBL; BENTA; BIMP3; IMD11; CARMA1
  [Similar Products]
NCBI Protein Information
caspase recruitment domain-containing protein 11; carma 1; CARD-containing MAGUK protein 1; bcl10-interacting maguk protein 3
UniProt Protein Name
Caspase recruitment domain-containing protein 11
UniProt Synonym Protein Names
CARD-containing MAGUK protein 1; Carma 1
UniProt Gene Name
CARD11  [Similar Products]
UniProt Synonym Gene Names
CARMA1; Carma 1  [Similar Products]
UniProt Entry Name
CAR11_HUMAN
NCBI Summary for CARD11
The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. [provided by RefSeq, Jul 2008]
UniProt Comments for CARD11
CARD11: a CARD (caspase recruitment domain) protein that belongs to the membrane-associated guanylate kinase (MAGUK) family. MAGUK proteins function as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. Contains an N-terminal CARD domain, a central coiled-coil domain, and a C-terminal region containing a PDZ, an SH3, and a GUK. Its CARD domain associates with the CARD domain of BCL10. BCL10 activates NF-kappaB through the IkappaB kinase complex in response to upstream stimuli. Apparently activates NF-kappaB and induce the phosphorylation of BCL10. Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; immunological synapse; cytosol; lipid raft

Molecular Function: guanylate kinase activity; protein binding; CARD domain binding

Biological Process: positive regulation of cytokine production; thymic T cell selection; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-2 biosynthetic process; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; regulation of apoptosis; regulation of B cell differentiation; T cell costimulation; positive regulation of B cell proliferation; innate immune response; regulation of T cell differentiation; positive regulation of T cell proliferation; nucleotide phosphorylation

Disease: Persistent Polyclonal B-cell Lymphocytosis; Immunodeficiency 11; B-cell Expansion With Nfkb And T-cell Anergy
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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