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anti-CD55 antibody :: anti-Human, Mouse CD55 Polyclonal Antibody

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Catalog # MBS178344
Unit / Price
  0.1 mg  /  $315 +1 FREE 8GB USB
Product Name

CD55, Polyclonal Antibody

Popular Item
Also Known As

Anti-CD55 Antibody

Product Synonym Names
Complement decay-accelerating factor; CD 55; CD55; CD55 antigen; CD55 Cromer blood group system; CD55 molecule; CD55 molecule, decay accelerating factor for complement (Cromer blood group); Cd55a; Complement decay accelerating factor; Complement decay-accelerating factor; Complement decay-accelerating factor, GPI-anchored; CR; CROM; Cromer Blood Group antigen; Cromer blood group system; DAF; Daf-GPI; DAF_HUMAN; Daf1; Dcay accelerating factor for complement (CD55, Cromer blood group system); Decay accelarating factor 1, isoform CRA_a; Decay accelerating factor (GPI-form); Decay Accelerating Factor for Complement; Decay accelerating factor GPI-form; Decay accelerating factor soluble-form; GPI-DAF; TC; CD55 molecule, decay accelerating factor for complement (Cromer blood group)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
381
OMIM
125240
3D Structure
ModBase 3D Structure for P08174
Clonality
Polyclonal
Species Reactivity
Human, Mouse
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized
Immunogen
E Coli-derived human CD55 recombinant protein (Position: D35-K347). Human CD55 shares 49.1% amino acid (aa) sequence identity with mouse CD55.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-CD55 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD55 antibody
Description: Rabbit IgG polyclonal antibody for Complement decay-accelerating factor(CD55) detection. Tested with WB, IHC-P in Human;Mouse.

Background: Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.
Applications Tested/Suitable for anti-CD55 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-CD55 antibody
Western Blot
Concentration: 0.1-0.5ug/ml
Tested Species: Hu, Ms

Immunohistochemistry (IHC) Paraffin:
Concentration: 0.5-1ug/ml
Tested Species: Hu
Antigen Retrieval: By Heat

Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-CD55 antibody
Anti- CD55 Picoband antibody, MBS178344, Western blotting
All lanes: Anti CD55 (MBS178344) at 0.5ug/ml
WB: K562 Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
anti-CD55 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-CD55 antibody
Anti- CD55 Picoband antibody, MBS178344, IHC(P)
IHC(P): Human Placenta Tissue
anti-CD55 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CD55. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
59,038 Da
NCBI Official Full Name
complement decay-accelerating factor isoform 1 preproprotein
NCBI Official Synonym Full Names
CD55 molecule (Cromer blood group)
NCBI Official Symbol
NCBI Official Synonym Symbols
CR; TC; DAF; CROM
  [Similar Products]
NCBI Protein Information
complement decay-accelerating factor
UniProt Protein Name
Complement decay-accelerating factor
UniProt Synonym Protein Names
CD_antigen: CD55
UniProt Gene Name
UniProt Synonym Gene Names
CR; DAF  [Similar Products]
UniProt Entry Name
DAF_HUMAN
NCBI Summary for CD55
This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014]
UniProt Comments for CD55
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
Product References and Citations for anti-CD55 antibody
1. Medof ME, Lublin DM, Holers VM, Ayers DJ, Getty RR, Leykam JF, Atkinson JP, Tykocinski ML (April 1987). "Cloning and characterization of cDNAs encoding the complete sequence of decay-accelerating factor of human complement". Proc. Natl. Acad. Sci. U.S.A. 84 (7): 2007-11.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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