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anti-CDK5R1 antibody :: Rabbit anti-Human, mouse CDK5R1 Polyclonal Antibody

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Catalog # MBS9213336
Unit / Price
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  0.08 mL  /  $150 +1 FREE 8GB USB
Western Blot (WB)
Product Name

CDK5R1, Polyclonal Antibody

Popular Item
Full Product Name

CDK5R1 Antibody (N-term)

Product Synonym Names
Cyclin-dependent kinase 5 activator 1; CDK5 activator 1; Cyclin-dependent kinase 5 regulatory subunit 1; TPKII regulatory subunit; Cyclin-dependent kinase 5 activator 1; p35; p35; Cyclin-dependent kinase 5 activator 1; p25; p25; Tau protein kinase II 23 kDa subunit; p23; CDK5R1; CDK5R; NCK5A
Antibody/Peptide Pairs
CDK5R1 peptide (MBS9230056) is used for blocking the activity of CDK5R1 antibody (MBS9213336)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
603460
3D Structure
ModBase 3D Structure for Q15078
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human, mouse
Specificity
This CDK5R1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 30-64 amino acids from the N-terminal region of human CDK5R1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.309 (lot specific)
Cellular Location
Cyclin-dependent kinase 5 activator 1, p35: Cell membrane; Lipid-anchor; Cytoplasmic side. Note=In the primary cortical neurons, p35 is present in the peripheries and nerve terminals
Tissue Location
Brain and neuron specific.
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-CDK5R1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CDK5R1 antibody
p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer in association with altered stability and subcellular distribution.
Applications Tested/Suitable for anti-CDK5R1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-CDK5R1 antibody
WB~~1:2000

Western Blot (WB) of anti-CDK5R1 antibody
All lanes : Anti-CDK5R1 Antibody (N-term) at 1:2000 dilution
Lane 1: human cerebellum lysates
Lane 2: mouse brain lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 34 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-CDK5R1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for CDK5R1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
34060
NCBI Official Full Name
cyclin-dependent kinase 5 activator 1
NCBI Official Synonym Full Names
cyclin-dependent kinase 5, regulatory subunit 1 (p35)
NCBI Official Symbol
CDK5R1  [Similar Products]
NCBI Official Synonym Symbols
p23; p25; p35; CDK5R; NCK5A; CDK5P35; p35nck5a
  [Similar Products]
NCBI Protein Information
cyclin-dependent kinase 5 activator 1
UniProt Protein Name
Cyclin-dependent kinase 5 activator 1
UniProt Synonym Protein Names
Cyclin-dependent kinase 5 regulatory subunit 1; TPKII regulatory subunit
UniProt Gene Name
CDK5R1  [Similar Products]
UniProt Synonym Gene Names
CDK5R; NCK5A; CDK5 activator 1; p35; p25; p23  [Similar Products]
UniProt Entry Name
CD5R1_HUMAN
NCBI Summary for CDK5R1
The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq, Jul 2008]
UniProt Comments for CDK5R1
CDK5R1: activator of cyclin-dependent kinase 5 (CDK5). Required for proper development of the central nervous system. The p35 form of this protein is cleaved by calpain, generating a p25 form, resulting in the prolonged activation and relocalization of the CDK5 from the cell periphery to nuclear and perinuclear regions. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease, leading to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: contractile fiber; intracellular membrane-bound organelle; cyclin-dependent protein kinase 5 activator complex; postsynaptic density; dendrite; dendritic spine; cytosol; nucleoplasm; growth cone; cell soma; membrane; axon; perinuclear region of cytoplasm; cytoplasm; plasma membrane; neuromuscular junction; nucleus

Molecular Function: protein serine/threonine kinase activator activity; protein binding; ephrin receptor binding; cyclin-dependent protein kinase activity; cadherin binding; cyclin-dependent protein kinase 5 activator activity; kinase activity; calcium ion binding; protein kinase binding; protein kinase activity

Biological Process: superior olivary nucleus maturation; axon guidance; regulation of neuron differentiation; rhythmic process; neuron migration; negative regulation of axon extension; neuron differentiation; positive regulation of neuron apoptosis; ephrin receptor signaling pathway; cerebellum development; neuron adhesion; neurite development; layer formation in the cerebral cortex; serine phosphorylation of STAT3 protein; acetylcholine receptor signaling, muscarinic pathway; hippocampus development; peptidyl-threonine phosphorylation; axonal fasciculation; cell proliferation; peptidyl-serine phosphorylation; embryonic development; ionotropic glutamate receptor signaling pathway; regulation of actin cytoskeleton organization and biogenesis; regulation of cyclin-dependent protein kinase activity; brain development; negative regulation of transcription, DNA-dependent
Product References and Citations for anti-CDK5R1 antibody
Tsai L.-H.,et al.Nature 371:419-423(1994).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Patrick G.N.,et al.Nature 402:615-622(1999).
Kerokoski P.,et al.Brain Res. Mol. Brain Res. 106:50-56(2002).

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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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