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anti-CHML antibody :: Rabbit anti-Human, Mouse CHML Polyclonal Antibody

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Catalog # MBS9605823
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

CHML, Polyclonal Antibody

Popular Item
Full Product Name

CHML Antibody

Product Synonym Names
CHM like; Chml; Choroideraemia like protein; Choroideraemia-like protein; Choroideremia like (Rab escort protein 2); Choroideremia like; Choroideremia like protein; Rab escort protein 2; Rab proteins geranylgeranyltransferase component A 2; RAE2_HUMAN; REP 2; REP-2; REP2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P26374
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Specificity
CHML antibody detects endogenous levels of total CHML
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide
Subcellular Location
Cytosol
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-CHML antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CHML antibody
Function: Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than CHM in supporting prenylation of Rab3 family.
Subunit Structure: Monomer. Heterotrimer composed of RABGGTA, RABGGTB and CHML; within this trimer, RABGGTA and RABGGTB form the catalytic component B, while CHML (component A) mediates Rab protein binding. Interacts with RAB1A, RAB7A and RAB27A, but has much lower affinity for RAB1A, RAB7A and RAB27A than CHM.
Similarity: Belongs to the Rab GDI family.
Applications Tested/Suitable for anti-CHML antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-CHML antibody
WB: 1:500-1:1000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500

Immunohistochemistry (IHC) of anti-CHML antibody
MBS9605823 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-CHML antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-CHML antibody
Western blot analysis of extracts from 293, using CHML Antibody. Lane 1 was treated with the blocking peptide.
anti-CHML antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-CHML antibody
MBS9605823 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
anti-CHML antibody Immunofluorescence (IF) image
Western Blot (WB) of anti-CHML antibody
Western blot analysis of extracts from COLO cells, using CHML antibody.The lane on the left is treated with the antigen-specific peptide.
anti-CHML antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for CHML. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 85 kD
Predicted: 75 kDa
NCBI Official Full Name
rab proteins geranylgeranyltransferase component A 2
NCBI Official Synonym Full Names
CHM like, Rab escort protein 2
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
rab proteins geranylgeranyltransferase component A 2
UniProt Protein Name
Rab proteins geranylgeranyltransferase component A 2
UniProt Synonym Protein Names
Choroideremia-like protein; Rab escort protein 2; REP-2
UniProt Gene Name
UniProt Synonym Gene Names
REP2; REP-2  [Similar Products]
NCBI Summary for CHML
The product of the CHML gene supports geranylgeranylation of most Rab proteins and may substitute for REP-1 in tissues other than retina. CHML is localized close to the gene for Usher syndrome type II. [provided by RefSeq, Jul 2008]
UniProt Comments for CHML
Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than CHM in supporting prenylation of Rab3 family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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