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anti-CLK4 antibody :: Rabbit anti-Human, Mouse CLK4 Polyclonal Antibody

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Catalog # MBS9608962
Unit / Price
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  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

CLK4, Polyclonal Antibody

Popular Item
Full Product Name

CLK4 Antibody

Product Synonym Names
AI987988; C85119; CDC like kinase 4; CDC-like kinase 4; CLK4; CLK4_HUMAN; DKFZp686A20267; Dual specificity protein kinase CLK4; OTTMUSP00000005756; OTTMUSP00000005757; OTTMUSP00000041113; Protein serine threonine kinase Clk4; RP23-9N7.1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9HAZ1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Specificity
CLK4 antibody detects endogenous levels of total CLK4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human CLK4
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in liver, kidney, heart, muscle, brain and endothelial cells.
Predicted Cross Reactivity
Bovine, Horse, Sheep, Dog
Similarity
Bovine (100%), Horse (100%), Sheep (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-CLK4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CLK4 antibody
Function: Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine-and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates SRSF1 and SRSF3. Required for the regulation of alternative splicing of MAPT/TAU. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
Subunit Structure: Interacts with UBL5.
Post-translational Modifications: Autophosphorylates on all three types of residues.
Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
Applications Tested/Suitable for anti-CLK4 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-CLK4 antibody
WB: 1:1000-3000
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-CLK4 antibody
MBS9608962 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
anti-CLK4 antibody Immunofluorescence (IF) image
Western Blot (WB) of anti-CLK4 antibody
Western blot analysis of CLK4 using COLO205 whole cell lysates
anti-CLK4 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for CLK4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 57 kDa
Predicted: 58 kDa
NCBI Official Full Name
dual specificity protein kinase CLK4
NCBI Official Synonym Full Names
CDC like kinase 4
NCBI Official Symbol
NCBI Protein Information
dual specificity protein kinase CLK4
UniProt Protein Name
Dual specificity protein kinase CLK4
UniProt Synonym Protein Names
CDC-like kinase 4
UniProt Gene Name
NCBI Summary for CLK4
The protein encoded by this gene belongs to the CDC2-like protein kinase (CLK) family. This protein kinase can interact with and phosphorylate the serine- and arginine-rich (SR) proteins, which are known to play an important role in the formation of spliceosomes, and thus may be involved in the regulation of alternative splicing. Studies in the Israeli sand rat Psammomys obesus suggested that the ubiquitin-like 5 (UBL5/BEACON), a highly conserved ubiquitin-like protein, may interact with and regulate the activity of this kinase. Multiple alternatively spliced transcript variants have been observed, but the full-length natures of which have not yet been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for CLK4
Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates SRSF1 and SRSF3. Required for the regulation of alternative splicing of MAPT/TAU. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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