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anti-COL9A1 antibody :: Rabbit anti-Human, Mouse Collagen IX alpha1 Polyclonal Antibody

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Catalog # MBS9609221
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

Collagen IX alpha1 (COL9A1), Polyclonal Antibody

Popular Item
Also Known As

Collagen IX alpha1 Antibody

Product Synonym Names
alpha 1 IX collagen chain; Alpha 1 type IX collagen; alpha-1(IX) collagen chain; Cartilage specific short collagen; Collagen alpha 1 IX chain; Collagen alpha-1(IX) chain; Collagen cartilage-specific short alpha collagen; Collagen cartilage-specific short alpha-1(IX) collagen chain; Collagen IX alpha 1 polypeptide; Collagen IX, alpha 1 polypeptide; collagen, type IX alpha; collagen, type IX, alpha 1; DJ149L1.1.2; EDM 6; EDM6; FLJ40263; MED; STL 4; STL4
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
3D Structure
ModBase 3D Structure for P20849
Species Reactivity
Human, Mouse
Collagen IX alpha1 antibody detects endogenous levels of total Collagen IX alpha1
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
1mg/ml (lot specific)
A synthesized peptide derived from human Collagen IX alpha1
Subcellular Location
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-COL9A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-COL9A1 antibody
Function: Structural component of hyaline cartilage and vitreous of the eye.
Subunit Structure: Heterotrimer of an alpha 1(IX), an alpha 2(IX) and an alpha 3(IX) chain.
Post-translational Modifications: Covalently linked to the telopeptides of type II collagen by lysine-derived cross-links. Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Similarity: Each subunit is composed of three triple-helical domains interspersed with non-collagenous domains. The globular domain at the N-terminus of type IX collagen molecules represents the NC4 domain which may participate in electrostatic interactions with polyanionic glycosaminoglycans in cartilage. Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.
Applications Tested/Suitable for anti-COL9A1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-COL9A1 antibody
WB: 1:1000-3000
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-COL9A1 antibody
MBS9609221 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
anti-COL9A1 antibody Immunofluorescence (IF) image
Western Blot (WB) of anti-COL9A1 antibody
Western blot analysis of Collagen IX alpha1 using LOVO whole cell lysates
anti-COL9A1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for COL9A1. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 92 kDa
Predicted: 92 kDa
NCBI Official Full Name
collagen alpha-1(IX) chain isoform 1
NCBI Official Synonym Full Names
collagen type IX alpha 1 chain
NCBI Official Symbol
COL9A1  [Similar Products]
NCBI Official Synonym Symbols
MED; EDM6; STL4; DJ149L1.1.2
  [Similar Products]
NCBI Protein Information
collagen alpha-1(IX) chain
UniProt Protein Name
Collagen alpha-1(IX) chain
Protein Family
UniProt Gene Name
COL9A1  [Similar Products]
NCBI Summary for COL9A1
This gene encodes one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule, and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in this gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects. Two transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for COL9A1
Structural component of hyaline cartilage and vitreous of the eye.
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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