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anti-DAD1 antibody :: Rabbit anti-Human, Mouse DAD1 Polyclonal Antibody

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Catalog # MBS151036
Unit / Price
  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

DAD1, Polyclonal Antibody

Also Known As

DAD1 Antibody

Product Synonym Names
DAD1; OST2; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; Defender against cell death 1; Oligosaccharyl transferase subunit DAD1; defender against cell death 1
Antibody/Peptide Pairs
DAD1 peptide (MBS152867) is used for blocking the activity of DAD1 antibody (MBS151036)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
113
OMIM
600243
3D Structure
ModBase 3D Structure for P61803
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
DAD1 Antibody is Ion exchange chromatography purified.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
DAD1 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1.
Buffer
DAD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
DAD1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-DAD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DAD1 antibody
DAD1 Antibody: Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.
Applications Tested/Suitable for anti-DAD1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-DAD1 antibody
DAD1 antibody can be used for detection of DAD1 by Western blot at 0.5 to 2 mug/mL. Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 10 mug/mL.

Western Blot (WB) of anti-DAD1 antibody
Western blot analysis of DAD1 in HepG2 cell lysate with DAD1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
anti-DAD1 antibody Western Blot (WB) (WB) image
Immunocytochemistry (ICC) of anti-DAD1 antibody
Immunocytochemistry of DAD1 in HepG2 cells with DAD1 antibody at 10 μg/mL.
anti-DAD1 antibody Immunocytochemistry (ICC) (ICC) image
Immunofluorescence (IF) of anti-DAD1 antibody
Immunofluorescence of DAD1 in HepG2 cells with DAD1 antibody at 10 μg/mL.
anti-DAD1 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for DAD1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
12,497 Da
NCBI Official Full Name
Defender against cell death 1
NCBI Official Synonym Full Names
defender against cell death 1
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; DAD-1; oligosaccharyltransferase 2 homolog; oligosaccharyl transferase subunit DAD1; oligosaccharyltransferase subunit 2 (non-catalytic)
UniProt Protein Name
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
UniProt Synonym Protein Names
Defender against cell death 1; DAD-1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Oligosaccharyl transferase subunit DAD1; DAD-1  [Similar Products]
UniProt Entry Name
DAD1_HUMAN
NCBI Summary for DAD1
DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008]
UniProt Comments for DAD1
DAD1: Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis. Belongs to the DAD/OST2 family.

Protein type: Endoplasmic reticulum; Transferase; Glycan Metabolism - N-glycan biosynthesis; Membrane protein, integral; Apoptosis; Membrane protein, multi-pass; EC 2.4.99.18

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: endoplasmic reticulum membrane; membrane; oligosaccharyl transferase complex; integral to membrane

Molecular Function: dolichyl-diphosphooligosaccharide-protein glycotransferase activity; oligosaccharyl transferase activity

Biological Process: response to drug; blastocyst development; cellular protein metabolic process; apoptosis; protein amino acid glycosylation; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; response to nutrient; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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