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anti-DEPDC5 antibody :: Rabbit DEPD5 Polyclonal Antibody

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Catalog # MBS9207358
Unit / Price
  0.08 mL  /  $165 +1 FREE 8GB USB
  0.4 mL  /  $370 +1 FREE 8GB USB
Product Name

DEPD5 (DEPDC5), Polyclonal Antibody

Popular Item
Full Product Name

DEPD5 Antibody (C-term)

Product Synonym Names
DEP domain-containing protein 5; DEPDC5; KIAA0645
Antibody/Peptide Pairs
DEPD5 peptide (MBS9218794) is used for blocking the activity of DEPD5 antibody (MBS9207358)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
1404-1432
OMIM
604364
3D Structure
ModBase 3D Structure for O75140
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human (Predicted Reactivity: Mouse)
Specificity
This DEPD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1404-1432 amino acids of human DEPD5.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.49 (lot specific)
Antigen Type
Synthetic Peptide
Clone Names
RB31640
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-DEPDC5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-DEPDC5 antibody
Applications Tested/Suitable for anti-DEPDC5 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes for anti-DEPDC5 antibody
FC~~1:25
WB~~1:2000

Flow Cytometry of anti-DEPDC5 antibody
Overlay histogram showing Hela cells stained with MBS9207358 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9207358, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
anti-DEPDC5 antibody Flow Cytometry (FC/FACS) image
Western Blot of anti-DEPDC5 antibody
All lanes : Anti-DEPD5 Antibody (C-term) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: KG-1 whole cell lysate Lane 3: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 171 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
anti-DEPDC5 antibody Western Blot (WB) image
NCBI/Uniprot data below describe general gene information for DEPDC5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
181264
NCBI Official Full Name
DEP domain-containing protein 5 isoform 2
NCBI Official Synonym Full Names
DEP domain containing 5
NCBI Official Symbol
DEPDC5  [Similar Products]
NCBI Official Synonym Symbols
DEP.5; FFEVF
  [Similar Products]
NCBI Protein Information
DEP domain-containing protein 5
UniProt Protein Name
DEP domain-containing protein 5
UniProt Gene Name
DEPDC5  [Similar Products]
UniProt Synonym Gene Names
KIAA0645  [Similar Products]
UniProt Entry Name
DEPD5_HUMAN
NCBI Summary for DEPDC5
This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
UniProt Comments for DEPDC5
DEPDC5: Belongs to the IML1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: perinuclear region of cytoplasm; lysosomal membrane; cytosol

Molecular Function: GTPase activator activity

Biological Process: positive regulation of GTPase activity

Disease: Epilepsy, Familial Focal, With Variable Foci
Product References and Citations for anti-DEPDC5 antibody
Seng, T.J., et al. Genes Chromosomes Cancer 43(2):181-193(2005)
Dunham, I., et al. Nature 402(6761):489-495(1999)

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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