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anti-ERK8 antibody :: Rabbit anti-Human, Mouse ERK8 Polyclonal Antibody

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Catalog # MBS9600010
Unit / Price
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  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

ERK8, Polyclonal Antibody

Popular Item
Full Product Name

Phospho-ERK8(Thr175+Tyr177) Antibody

Product Synonym Names
ERK-7; ERK-8; ERK7; ERK8; Extracellular regulated kinase 8 delta; Extracellular signal regulated kinase 8; Extracellular signal-regulated kinase 7; Extracellular signal-regulated kinase 8; MAP kinase 15; MAPK 15; Mapk15; Mitogen activated protein kinase 15; Mitogen-activated protein kinase 15; MK15_HUMAN
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8TD08
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Specificity
Phospho-ERK8(Thr175+Tyr177) antibody detects endogenous levels of ERK8 only when phosphorylated at Threonine 175 and Tyrosine 177
Purity/Purification
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human ERK8 around the phosphorylation site of Threonine 175 and Tyrosine 177
Tissue Specificity
Widely expressed with a maximal expression in lung and kidney.
Conjugation
Unconjugated
Epitope
Phospho Thr175, Phospho Tyr177
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-ERK8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ERK8 antibody
Description: ERK7 is phosphorylates MBP in vitro. Interacts with CSK/c-Src, ABL1 and RET. 3 isoforms of the human protein are produced by alternative splicing.
Function: In vitro, phosphorylates MBP.
Subunit Structure: Interacts with CSK/c-Src, ABL1, RET and TGFB1I1.
Post-translational Modifications: Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro. Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase (By similarity).
Similarity: The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation.The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Applications Tested/Suitable for anti-ERK8 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-ERK8 antibody
WB: 1:500-1:2000
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-ERK8 antibody
MBS9600010 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
anti-ERK8 antibody Immunofluorescence (IF) image
Western Blot (WB) of anti-ERK8 antibody
Western blot analysis on Jurkat cell lysate using Phospho-ERK8(Thr175+Tyr177) Antibody, The lane on the left is treated with the antigen-specific peptide.
anti-ERK8 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ERK8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 60 kDa
Predicted: 60 kDa
NCBI Official Full Name
mitogen-activated protein kinase 15
NCBI Official Synonym Full Names
mitogen-activated protein kinase 15
NCBI Official Symbol
MAPK15  [Similar Products]
NCBI Official Synonym Symbols
ERK7; ERK8
  [Similar Products]
NCBI Protein Information
mitogen-activated protein kinase 15
UniProt Protein Name
Mitogen-activated protein kinase 15
UniProt Synonym Protein Names
Extracellular signal-regulated kinase 7; ERK-7; Extracellular signal-regulated kinase 8; ERK-8
UniProt Gene Name
MAPK15  [Similar Products]
UniProt Synonym Gene Names
MAP kinase 15; MAPK 15; ERK-7; ERK-8  [Similar Products]
UniProt Comments for ERK8
Atypical MAPK protein that regulates several process such as autophagy, ciliogenesis, protein trafficking/secretion and genome integrity, in a kinase activity-dependent manner (PubMed:22948227, PubMed:24618899, PubMed:29021280, PubMed:21847093, PubMed:20733054). Controls both, basal and starvation-induced autophagy throught its interaction with GABARAP, MAP1LC3B and GABARAPL1 leading to autophagosome formation, SQSTM1 degradation and reduced MAP1LC3B inhibitory phosphorylation (PubMed:22948227). Regulates primary cilium formation and the localization of ciliary proteins involved in cilium structure, transport, and signaling (PubMed:29021280). Prevents the relocation of the sugar-adding enzymes from the Golgi to the endoplasmic reticulum, thereby restricting the production of sugar-coated proteins (PubMed:24618899). Upon amino-acid starvation, mediates transitional endoplasmic reticulum site disassembly and inhibition of secretion (PubMed:21847093). Binds to chromatin leading to MAPK15 activation and interaction with PCNA, that which protects genomic integrity by inhibiting MDM2-mediated degradation of PCNA (PubMed:20733054). Regulates DA transporter (DAT) activity and protein expression via activation of RhoA (PubMed:28842414). In response to H2O2 treatment phosphorylates ELAVL1, thus preventing it from binding to the PDCD4 3'UTR and rendering the PDCD4 mRNA accessible to miR-21 and leading to its degradation and loss of protein expression (PubMed:26595526). Also functions in a kinase activity-independent manner as a negative regulator of growth (). Phosphorylates in vitro FOS and MBP (PubMed:11875070, PubMed:16484222, PubMed:20638370, PubMed:19166846). During oocyte maturation, plays a key role in the microtubule organization and meiotic cell cycle progression in oocytes, fertilized eggs, and early embryos (). Interacts with ESRRA promoting its re-localization from the nucleus to the cytoplasm and then prevents its transcriptional activity (PubMed:21190936).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Products associated with anti-ERK8 antibody
 Reference Product  PubMed Publications
 JUN antibody  >1 publications with ERK8 and JUN
Organs/Tissues associated with anti-ERK8 antibody
 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >1 publications with ERK8 and Pancreas
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