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anti-ERO1B antibody :: Rabbit anti-Human, mouse ERO1B Polyclonal Antibody

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Catalog # MBS7003509
Unit / Price
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

ERO1B, Polyclonal Antibody

Popular Item
Full Product Name

ERO1B Antibody

Product Synonym Names
ERO1-like protein beta; ERO1-L-beta; Endoplasmic reticulum oxidoreductase beta; ERO1B
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
615437
3D Structure
ModBase 3D Structure for Q86YB8
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, mouse
Purity/Purification
>95%,Protein G purified
Form/Format
Liquid
Immunogen
Recombinant human ERO1-like protein beta protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-ERO1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ERO1B antibody
Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Other protein disulfide isomerase family members can also be reoxidized, but at lower rates compared to P4HB, including PDIA2 (50% of P4HB reoxidation rate), as well as PDIA3, PDIA4, PDIA6 and NXNDC12 (<10%). Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. May be involved in oxidative proinsulin folding in pancreatic cells, hence may play a role in glucose homeostasis.
Applications Tested/Suitable for anti-ERO1B antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Western Blot (WB) of anti-ERO1B antibody
Western blot
All lanes: ERO1B antibody at 3.2ug/ml
Lane 1: MCF7 whole cell lysate
Lane 2: U251 whole cell lysate
Lane 3: Mouse liver tissue
Lane 4: U87 whole cell lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 54,17 kDa
Observed band size: 54 kDa

anti-ERO1B antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ERO1B antibody
Immunohistochemistry of paraffin-embedded human pancreatic tissue using MBS7003509 at dilution of 1:100
anti-ERO1B antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ERO1B antibody
Immunohistochemistry of paraffin-embedded human pancreatic cancer using MBS7003509 at dilution of 1:100
anti-ERO1B antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ERO1B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
16,096 Da
NCBI Official Full Name
ERO1-like protein beta
NCBI Official Synonym Full Names
endoplasmic reticulum oxidoreductase beta
NCBI Official Symbol
ERO1B  [Similar Products]
NCBI Official Synonym Symbols
ERO1LB; Ero1beta
  [Similar Products]
NCBI Protein Information
ERO1-like protein beta
UniProt Protein Name
ERO1-like protein beta
UniProt Synonym Protein Names
Endoplasmic reticulum oxidoreductase beta
Protein Family
UniProt Gene Name
ERO1B  [Similar Products]
UniProt Synonym Gene Names
ERO1-L-beta  [Similar Products]
UniProt Entry Name
ERO1B_HUMAN
UniProt Comments for ERO1B
ERO1LB: Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the being a source of oxidative stress. Required for the folding of cell, thereby being a source of oxidative stress. Belongs to the EROs family.

Protein type: Oxidoreductase; Endoplasmic reticulum; EC 1.8.4.-

Chromosomal Location of Human Ortholog: 1q42.2-q43

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane

Molecular Function: oxidoreductase activity; oxidoreductase activity, acting on sulfur group of donors, disulfide as acceptor; protein binding; protein disulfide isomerase activity; protein disulfide oxidoreductase activity; unfolded protein binding

Biological Process: 4-hydroxyproline metabolic process; cell redox homeostasis; extracellular matrix organization and biogenesis; glucose homeostasis; insulin processing; protein folding; protein maturation via protein folding
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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