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anti-F12 antibody :: Rabbit anti-Human, Mouse Factor XII HC Polyclonal Antibody

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Catalog # MBS8242193
Unit / Price
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  0.03 mL  /  $155 +1 FREE 8GB USB
  0.1 mL  /  $220 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

Factor XII HC (F12), Polyclonal Antibody

Popular Item
Full Product Name

Anti-Factor XII HC Antibody

Product Synonym Names
Coagulation factor XII; Hageman factor; HAF
Product Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
234000
3D Structure
ModBase 3D Structure for P00748
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Specificity
Recognizes endogenous levels of Factor XII HC protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Factor XII HC. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-F12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-F12 antibody
Rabbit polyclonal antibody to Factor XII HC
Applications Tested/Suitable for anti-F12 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-F12 antibody
Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200)

Western Blot (WB) of anti-F12 antibody
Western blot analysis of Factor XII HC expression in MDAMB435 (A), NIH3T3 (B) whole cell lysates.
anti-F12 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-F12 antibody
Immunohistochemical analysis of Factor XII HC staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-F12 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for F12. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
67,792 Da
NCBI Official Full Name
Coagulation factor XII
NCBI Official Synonym Full Names
coagulation factor XII
NCBI Official Symbol
NCBI Official Synonym Symbols
HAF; HAE3; HAEX
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NCBI Protein Information
coagulation factor XII
UniProt Protein Name
Coagulation factor XII
UniProt Synonym Protein Names
Hageman factor; HAF
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
FA12_HUMAN
NCBI Summary for F12
This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. [provided by RefSeq, Jul 2008]
UniProt Comments for F12
F12: Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta- factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa. Defects in F12 are the cause of factor XII deficiency (FA12D); also known as Hageman factor deficiency. This trait is an asymptomatic anomaly of in vitro blood coagulation. Its diagnosis is based on finding a low plasma activity of the factor in coagulating assays. It is usually only accidentally discovered through pre-operative blood tests. F12 deficiency is divided into two categories, a cross-reacting material (CRM)-negative group (negative F12 antigen detection) and a CRM-positive group (positive F12 antigen detection). Defects in F12 are the cause of hereditary angioedema type 3 (HAE3); also known as estrogen-related HAE or hereditary angioneurotic edema with normal C1 inhibitor concentration and function. HAE is characterized by episodic local subcutaneous edema, and submucosal edema involving the upper respiratory and gastrointestinal tracts. HAE3 occurs exclusively in women and is precipitated or worsened by high estrogen levels (e.g. during pregnancy or treatment with oral contraceptives). It differs from HAE types 1 and 2 in that both concentration and function of C1 inhibitor are normal. Belongs to the peptidase S1 family.

Protein type: Protease; Secreted; Chaperone; EC 3.4.21.38; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: extracellular region; extracellular space; plasma membrane

Molecular Function: misfolded protein binding; protein binding; serine-type endopeptidase activity

Biological Process: blood coagulation; blood coagulation, intrinsic pathway; Factor XII activation; fibrinolysis; innate immune response; plasma kallikrein-kinin cascade; positive regulation of blood coagulation; positive regulation of fibrinolysis; protein autoprocessing; protein processing; response to misfolded protein; zymogen activation

Disease: Angioedema, Hereditary, Type Iii; Factor Xii Deficiency
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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