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anti-FLT3LG antibody :: Rabbit anti-Human, Mouse Flt3, Cytoplasmic Polyclonal Antibody

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Catalog # MBS616026
Unit / Price
  0.2 mg  /  $615 +1 FREE 8GB USB
WB
Product Name

Flt3, Cytoplasmic (FLT3LG), Polyclonal Antibody

Popular Item
Also Known As

Flt3, Cytoplasmic (Fms-like Tyrosine Kinase 3, CD135)

Product Synonym Names
Anti -Flt3, Cytoplasmic (Fms-like Tyrosine Kinase 3, CD135)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_006583.3 (107015222..107025096, complement). Location: chromosome: 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Specificity
Recognizes the processed (130kD) and the mature forms (155kD) of mouse Flt3. Species Crossreactivity: human
Purity/Purification
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
Concentration
1 mg/ml (lot specific)
Immunogen
His-tag fusion protein corresponding to aa786-1000 of mouse Flt3.
Grade
Affinity Purified
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of anti-FLT3LG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-FLT3LG antibody
Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in vitro in cooperation with other cytokines. Flt3 is also expressed in the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells.
Applications Tested/Suitable for anti-FLT3LG antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes for anti-FLT3LG antibody
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Dilution: Western Blot: 0.5-2ug/ml detected Flt3 in RIPA lysates from HeLa cells and mouse Bafl-3 cells. Bafl-3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with F4600-08 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitated Flt3 from 500ug of mouse Bafl-3 RIPA lysate.
Immunocytochemistry: 10ug/ml showed positive immunostaining for Flt3 in mouse Bafl-3 cells fixed with methanol and permeabilized with 0.25% Triton X-100 for 5 mins.

Balf-3 cell lysate was probed with MbS616026 at 1ug/ml
anti-FLT3LG antibody WB image
NCBI/Uniprot data below describe general gene information for FLT3LG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
fms-related tyrosine kinase 3 ligand
NCBI Official Symbol
FLT3LG  [Similar Products]
NCBI Protein Information
fms-related tyrosine kinase 3 ligand; Flt3 ligand
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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