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anti-TDG antibody :: Rabbit anti-Human, Mouse G/T mismatch-specific thymine DNA glycosylase Polyclonal Antibody

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Catalog # MBS1498143
Unit / Price
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  0.05 mL  /  $120 +1 FREE 8GB USB
  0.1 mL  /  $160 +1 FREE 8GB USB
Product Name

G/T mismatch-specific thymine DNA glycosylase (TDG), Polyclonal Antibody

Popular Item
Full Product Name

Rabbit anti-human G/T mismatch-specific thymine DNA glycosylase polyclonal Antibody(TDG)

Product Synonym Names
Thymine-DNA glycosylase; hTDG; TDG
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
601423
3D Structure
ModBase 3D Structure for Q13569
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Antigen Affinity Purified
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human G/T mismatch-specific thymine DNA glycosylase protein(141-410aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-22845 / sc-22847 / sc-292440
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-TDG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TDG antibody
DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.
Applications Tested/Suitable for anti-TDG antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-TDG antibody
Recommended dilution: WB:1:500-2000
IHC:1:20-1:200

Western Blot (WB) of anti-TDG antibody
All lanes: G/T mismatch-specific thymine DNA glycosylase antibody at 6ug/ml+mouse brain tissue
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 46kDa
Observed band size: 46kDa

anti-TDG antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-TDG antibody
Immunohistochemistry of paraffin-embedded human heart using MBS1498143 at dilution 1:100
anti-TDG antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-TDG antibody
Immunohistochemistry of paraffin-embedded human colon cancer using MBS1498143 at dilution 1:100
anti-TDG antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for TDG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46,053 Da
NCBI Official Full Name
G/T mismatch-specific thymine DNA glycosylase
NCBI Official Synonym Full Names
thymine DNA glycosylase
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
G/T mismatch-specific thymine DNA glycosylase
UniProt Protein Name
G/T mismatch-specific thymine DNA glycosylase
UniProt Synonym Protein Names
Thymine-DNA glycosylase; hTDG
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
TDG_HUMAN
NCBI Summary for TDG
The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008]
UniProt Comments for TDG
TDG: In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar- phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. Belongs to the TDG/mug DNA glycosylase family.

Protein type: EC 3.2.2.29; Hydrolase; DNA repair, damage; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: nucleoplasm; nucleus; plasma membrane; PML body

Molecular Function: damaged DNA binding; DNA N-glycosylase activity; double-stranded DNA binding; mismatched DNA binding; protein binding; protein homodimerization activity; protein kinase C binding; pyrimidine-specific mismatch base pair DNA N-glycosylase activity; structure-specific DNA binding; SUMO binding; uracil DNA N-glycosylase activity

Biological Process: base-excision repair; base-excision repair, AP site formation; cellular protein metabolic process; chromatin modification; depyrimidination; DNA repair; embryonic development; gene expression; mismatch repair; negative regulation of protein binding; negative regulation of transcription from RNA polymerase II promoter; post-translational protein modification; protein sumoylation; regulation of gene expression, epigenetic; transcription, DNA-dependent
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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