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anti-GLI2 antibody :: Goat GLI2 Polyclonal Antibody

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Catalog # MBS421392
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
anti-GLI2 antibody
Product Name

GLI2, Polyclonal Antibody

Full Product Name

Goat anti-GLI2 Antibody

Product Synonym Names
GLI2; GLI-Kruppel family member GLI2; THP2; oncogene GLI2; tax helper protein 2; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; zinc finger protein GLI2; GLI2 antibody; GLI-Kruppel family member GLI2 antibody; THP2 antibody; oncogene GLI2 antibody; tax helper protein 2 antibody; tax-responsive element-2 holding protein antibody; tax-responsive element-25-bp sequence binding protein antibody; zinc finger protein GLI2 antibody
Antibody/Peptide Pairs
GLI2 peptide (MBS425628) is used for blocking the activity of GLI2 antibody (MBS421392)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
DLKEDLDRDD CKQE
OMIM
165230
Clonality
Polyclonal
Host
Goat
Species Reactivity
Expected from sequence similarity: Human, Mouse
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence DLKEDLDRDDCKQE, from the internal region of the protein sequence according to NP_005261.2.
Epitope
Internal region
Note
This antibody is expected to recognise all four reported isoforms (NP_084655.1, NP_084656.1, NP_084657.1 and NP_005261.1)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-GLI2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-GLI2 antibody
Peptide ELISA (EIA)
Application Notes for anti-GLI2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 2000.
Western Blot: Preliminary experiments gave an approx 55kDa band in Human Bone Marrow and Ovary lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 168kDa according to NP_005261.2. The 55kDa band was successfully blocked by incubation with the immunizing peptide.
NCBI/Uniprot data below describe general gene information for GLI2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
86,482 Da
NCBI Official Full Name
zinc finger protein GLI2
NCBI Official Synonym Full Names
GLI family zinc finger 2
NCBI Official Symbol
NCBI Official Synonym Symbols
CJS; HPE9; PHS2; THP1; THP2
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NCBI Protein Information
zinc finger protein GLI2
UniProt Protein Name
Zinc finger protein GLI2
UniProt Synonym Protein Names
GLI family zinc finger protein 2
Protein Family
UniProt Gene Name
UniProt Entry Name
GLI2_HUMAN
NCBI Summary for GLI2
This gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008]
UniProt Comments for GLI2
Functions as transcription regulator in the hedgehog (Hh) pathway (PubMed:18455992). Functions as transcriptional activator (PubMed:9557682, PubMed:19878745, PubMed:24311597). May also function as transcriptional repressor (). Requires STK36 for full transcriptional activator activity. Required for normal embryonic development (PubMed:15994174, PubMed:20685856).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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