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anti-Irak2 antibody :: Rabbit anti-Human, Mouse IRAK2 Polyclonal Antibody

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Catalog # MBS150516
Unit / Price
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  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

IRAK2, Polyclonal Antibody

Full Product Name

IRAK2 Antibody

Product Synonym Names
IRAK2; IRAK-2; AI649099; 6330415L08Rik; Interleukin-1 receptor-associated kinase-like 2; IRAK-2; interleukin-1 receptor-associated kinase 2
Antibody/Peptide Pairs
IRAK2 peptide (MBS152293) is used for blocking the activity of IRAK2 antibody (MBS150516)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8CFA1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Specificity
Anti-IRAK2 has no cross response to IRAK. Anti-IRAK2 will also recognize human IRAK2.
Purity/Purification
IRAK2 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
IRAK2 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of mouse IRAK2a which is common to all four isoforms.
Buffer
IRAK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
IRAK2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-Irak2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Irak2 antibody
IRAK2 Antibody: The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-κB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family has been identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-κB activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. Unlike human IRAK2, murine IRAK2 exists as four alternately spliced isoforms (IRAK2a-d), with two isoforms (IRAK2c and d) acting in an inhibitory fashion. IRAK2 is expressed in a variety of tissues.
Applications Tested/Suitable for anti-Irak2 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-Irak2 antibody
IRAK2 antibody can be used for detection of mouse IRAK2 by Western blot at 0.5 - 2 mug/mL. A 65 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 1 mug/mL. For immunofluorescence start at 10 mug/mL.

Western Blot (WB) of anti-Irak2 antibody
Western blot analysis of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
anti-Irak2 antibody Western Blot (WB) (WB) image
Immunocytochemistry (ICC) of anti-Irak2 antibody
Immunocytochemistry of IRAK2 in A-20 cells with IRAK2 antibody at 1 μg/mL.
anti-Irak2 antibody Immunocytochemistry (ICC) (ICC) image
Immunofluorescence (IF) of anti-Irak2 antibody
Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 10 μg/mL.
anti-Irak2 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for Irak2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
52,463 Da
NCBI Official Full Name
interleukin-1 receptor-associated kinase 2a
NCBI Official Synonym Full Names
interleukin-1 receptor-associated kinase 2
NCBI Official Symbol
Irak2  [Similar Products]
NCBI Official Synonym Symbols
IRAK-2; AI649099; 6330415L08Rik
  [Similar Products]
NCBI Protein Information
interleukin-1 receptor-associated kinase-like 2; mu-IRAK-2; interleukin-1 receptor-associated kinase 2c
UniProt Protein Name
Interleukin-1 receptor-associated kinase-like 2
UniProt Gene Name
Irak2  [Similar Products]
UniProt Synonym Gene Names
IRAK-2; mu-IRAK-2  [Similar Products]
UniProt Entry Name
IRAK2_MOUSE
UniProt Comments for Irak2
IRAK2: Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Interacts with MYD88. IL-1 stimulation leads to the formation of a signaling complex which dissociates from the IL-1 receptor following the binding of PELI1. Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; IRAK family

Cellular Component: interleukin-1 receptor complex; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein homodimerization activity; protein heterodimerization activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; ATP binding; protein kinase activity

Biological Process: regulation of apoptosis; inhibition of NF-kappaB transcription factor; negative regulation of transcription factor activity; protein amino acid autophosphorylation; activation of NF-kappaB-inducing kinase; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; regulation of cytokine and chemokine mediated signaling pathway; signal transduction; protein amino acid phosphorylation; activation of NF-kappaB transcription factor
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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