• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-KSR1 antibody :: Rabbit anti-Human, Mouse KSR1 Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS9600686
Unit / Price
Scan QR to view Datasheet
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

KSR1, Polyclonal Antibody

Popular Item
Full Product Name

Phospho-KSR1 (Ser392/406) Antibody

Product Synonym Names
BKSR 1; BKSR1; dKsr; EK 31; EK31; hb; Kinase suppressor of Ras 1; Kinase suppressor of ras; KSR 1; KSR; KSR1; KSR1_HUMAN; Positive Ras signaling mediator family member (86.4 kD); Positive Ras signaling mediator family member; RSU 2; RSU2; SR 31; SR31; Suppressor of activated let 60 Ras SUR3; Suppressor of Ras1 31
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8IVT5
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Specificity
Phospho-KSR1 (Ser392/406) antibody detects endogenous levels of KSR1.
Purity/Purification
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human KSR1 around the phosphorylation site of Ser392/406.
Subcellular Location
Endoplasmic Reticulum; Plasma Membrane
Predicted Cross Reactivity
Pig, Bovine, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Rabbit (100%), Dog (100%), Chicken (92%)
Conjugation
Unconjugated
Epitope
Phospho Ser392, Phospho Ser406
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-KSR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-KSR1 antibody
Function: Scaffolding protein that is part of a multiprotein signaling complex. Promotes phosphorylation of Raf family members and activation of downstream MAP kinases. Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP. Does not have kinase activity by itself.
Subunit Structure: Interacts with HSP90AA1/HSP90, YWHAE/14-3-3, CDC37, MAP2K1/MEK-1 and MAP2K2/MEK-2 (PubMed:10409742). Interacts with MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization (By similarity). Interacts with isoform 1 of VRK2 (PubMed:20679487). Interacts with AKAP13 and BRAF (PubMed:21102438). Identified in a complex with AKAP13, MAP2K1 and BRAF (By similarity).
Post-translational Modifications: Phosphorylated on Ser-311 and, to a higher extent, on Ser-406 by MARK3. Dephosphorylated on Ser-406 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated. Phosphorylated by PKA at Ser-888. Phosphorylation at Ser-888 is required for cAMP-dependent activation of MAPK1 and/or MAPK3 (By similarity).
Similarity: The protein kinase domain is predicted to be catalytically inactive. The domain is sufficient for KSR1 and KSR1-mediated MAP2K1 and MAP2K2 membrane localization. The domain is required but not sufficient for MAP kinase-mediated inhibition of ELK1 phosphorylation (PubMed:10409742).The protein kinase domain is predicted to be catalytically inactive. The domain is sufficient for KSR1 and KSR1-mediated MAP2K1 and MAP2K2 membrane localization. The domain is required but not sufficient for MAP kinase-mediated inhibition of ELK1 phosphorylation.The N-terminal region mediates interaction with BRAF and with membranes. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Applications Tested/Suitable for anti-KSR1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-KSR1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Immunohistochemistry (IHC) of anti-KSR1 antibody
MBS9600686 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-KSR1 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-KSR1 antibody
Western blot analysis of extracts of HEK293 cells, using Phospho-KSR1 (Ser392/406) Antibody.
anti-KSR1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for KSR1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 115 kDa
Predicted: 103 kDa
NCBI Official Full Name
kinase suppressor of Ras 1
NCBI Official Synonym Full Names
kinase suppressor of ras 1
NCBI Official Symbol
NCBI Official Synonym Symbols
KSR; RSU2
  [Similar Products]
NCBI Protein Information
kinase suppressor of Ras 1
UniProt Protein Name
Kinase suppressor of Ras 1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Comments for KSR1
Scaffolding protein that is part of a multiprotein signaling complex. Promotes phosphorylation of Raf family members and activation of downstream MAP kinases. Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP. Does not have kinase activity by itself.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000