• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-STK4 antibody :: Rabbit anti-Human, Mouse Mst1,2 phosphorylated Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS614580
Unit / Price
  0.1 mL  /  $590 +1 FREE 8GB USB
anti-STK4 antibody
Product Name

Mst1,2 phosphorylated (STK4), Polyclonal Antibody

Full Product Name

Mst1,2 phosphorylated (Thr183,Thr180) (Mammalian Ste20-like Kinase)

Product Synonym Names
Anti -Mst1,2 phosphorylated (Thr183,Thr180) (Mammalian Ste20-like Kinase)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 20; NC_000020.10 (43595120..43708594). Location: 20q11.2-q13.2
OMIM
604965
3D Structure
ModBase 3D Structure for Q13043
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Specificity
Recognizes endogenous levels of human Thr183 phosphorylated Mst1 and Thr180 phosphorylated Mst2 at 50kD. This antibody may crossreact with phosphorylated Mst3 and Mst4. Species Crossreactivity
Purity/Purification
Affinity Purified
Protein A and peptide affinity chromatography
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol
Immunogen
Synthetic phospho-Thr183 peptide (KLH coupled) corresponding to residues surrounding threonine 183 of human Mst1.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-STK4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-STK4 antibody
Mammalian Ste20-like kinases, including Mst1-4, belong to the germinal center kinase (GCK) family. The amino-terminal kinase domain of Mst is considerably homologous to the kinase domain of yeast Ste20 kinase and other p21-activated kinases (1). The carboxy-terminal region of Mst1 and Mst2 contains a dimerization and an inhibitory domain, while the carboxy- terminal region of Mst3 and Mst4 are not well characterized (1-3). Dimerization and phosphorylation at the activation loop regulates translocation of Mst1 from the cytosol to the nucleus (3). Growing evidence indicates that Mst1, Mst2 and Mst3 are activated by apoptotic signals as well as other stress conditions (4-6). The full activation of Mst1 requires both phosphorylation and caspase-mediated cleavage (4). Sequence alignment of the activation loop of the GCK family indicates that Thr183 of Mst1 and Thr180 of Mst2 are the conserved residues and might be critical for the activity of the kinases. Activated Mst kinases may rely on p38 MAPK and JNK pathways to amplify apoptotic signals (5). Phosphorylation at serine 327 of Mst1, which is close to the caspase-3 recognization site, inhibits caspase-mediated cleavage (4).
Applications Tested/Suitable for anti-STK4 antibody
Western Blot (WB)
Application Notes for anti-STK4 antibody
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000
NCBI/Uniprot data below describe general gene information for STK4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
55,630 Da[Similar Products]
NCBI Official Full Name
MST1
NCBI Official Synonym Full Names
serine/threonine kinase 4
NCBI Official Symbol
NCBI Official Synonym Symbols
KRS2; MST1; YSK3; DKFZp686A2068
  [Similar Products]
NCBI Protein Information
serine/threonine-protein kinase 4; MST-1; OTTHUMP00000043418; OTTHUMP00000063226; STE20-like kinase MST1; mammalian sterile 20-like 1; kinase responsive to stress 2; mammalian STE20-like protein kinase 1; serine/threonine-protein kinase Krs-2; dJ211D12.2 (serine/threonine kinase 4 (MST1, KRS2))
UniProt Protein Name
Serine/threonine-protein kinase 4
UniProt Synonym Protein Names
Mammalian STE20-like protein kinase 1; MST-1; STE20-like kinase MST1; Serine/threonine-protein kinase Krs-2
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
STK4_HUMAN
NCBI Summary for STK4
The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq]
UniProt Comments for STK4
MST1: a protein kinase of the STE20 family. Proteolytically activated by caspase during apoptosis. Activated by apoptotic signals as well as other stress conditions. Full activation requires both phosphorylation and caspase-mediated cleavage. Phosphorylation at serine 327 of Mst1, which is close to the caspase-3 recognition site, inhibits caspase-mediated cleavage.

Protein type: Protein kinase, STE; Kinase, protein; Autophagy; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; STE group; STE20 family; MST subfamily

Chromosomal Location of Human Ortholog: 20q11.2-q13.2

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein dimerization activity; protein serine/threonine kinase activator activity; identical protein binding; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; magnesium ion binding; transcription factor binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: central nervous system development; positive regulation of protein binding; positive regulation of apoptosis; apoptosis; protein amino acid autophosphorylation; cell morphogenesis; negative regulation of organ growth; signal transduction; protein amino acid phosphorylation; patterning of blood vessels; keratinocyte differentiation; negative regulation of cell proliferation; peptidyl-serine phosphorylation; positive regulation of fat cell differentiation; neural tube formation

Disease: T-cell Immunodeficiency, Recurrent Infections, And Autoimmunity With Or Without Cardiac Malformations
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000