• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-STXBP1 antibody :: Goat MUNC18/STXBP1 Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS420125
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

MUNC18/STXBP1, Polyclonal Antibody

Also Known As

Goat anti-MUNC18/STXBP1 (isoform a) Antibody

Product Synonym Names
STXBP1; MUNC18-1; UNC18; hUNC18; syntaxin binding protein 1; Hunc18; rbSec1; syntaxin-binding protein 1; RP11-56D16.3; EIEE4; FLJ37475; RBSEC1; STXBP1 antibody; MUNC18-1 antibody; UNC18 antibody; hUNC18 antibody; syntaxin binding protein 1 antibody; Hunc18 antibody; rbSec1 antibody; syntaxin-binding protein 1 antibody; RP11-56D16.3 antibody; EIEE4 antibody; FLJ37475 antibody; RBSEC1 antibody; MUNC18 / STXBP1 (isoform a)
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
603
Immunogen Sequence
SRVSFEDQAP TME
OMIM
602926
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-SRVSFEDQAPTME, from the C Terminus of the protein sequence according to NP_003156.1.
Epitope
C Terminus
Note
This antibody is expected to recognize isoform a (NP_003156.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-STXBP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-STXBP1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-STXBP1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Brain cortex shows vesicular staining in neuronal bodies. Recommended concentration, 5-10ug/ml.
Western Blot: Approx 75kDa band observed in Human and Mouse Brain lysates (calculated MW of 68.7kDa according to NP_003156.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-STXBP1 antibody
(0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-STXBP1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-STXBP1 antibody
(5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-STXBP1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for STXBP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
68,736 Da
NCBI Official Full Name
syntaxin-binding protein 1 isoform a
NCBI Official Synonym Full Names
syntaxin binding protein 1
NCBI Official Symbol
STXBP1  [Similar Products]
NCBI Official Synonym Symbols
P67; NSEC1; UNC18; RBSEC1; MUNC18-1
  [Similar Products]
NCBI Protein Information
syntaxin-binding protein 1
UniProt Protein Name
Syntaxin-binding protein 1
UniProt Synonym Protein Names
MUNC18-1; N-Sec1; Protein unc-18 homolog 1; Unc18-1; Protein unc-18 homolog A; Unc-18A; p67
Protein Family
UniProt Gene Name
STXBP1  [Similar Products]
UniProt Synonym Gene Names
UNC18A; Unc18-1; Unc-18A  [Similar Products]
UniProt Entry Name
STXB1_HUMAN
NCBI Summary for STXBP1
This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010]
UniProt Comments for STXBP1
STXBP1: may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. Two splice-variant isoforms have been described.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: cytoplasm; cytosol; mitochondrion; myelin sheath; nucleoplasm; plasma membrane; protein complex; terminal button

Molecular Function: identical protein binding; protein binding; protein domain specific binding; protein kinase binding; protein N-terminus binding; SNARE binding; syntaxin binding; syntaxin-1 binding

Biological Process: axon target recognition; glutamate secretion; negative regulation of neuron apoptosis; negative regulation of synaptic transmission, GABAergic; neuromuscular synaptic transmission; neurotransmitter secretion; platelet degranulation; positive regulation of calcium ion-dependent exocytosis; protein stabilization; protein transport; regulation of synaptic vesicle fusion to presynaptic membrane; synaptic vesicle maturation; vesicle docking during exocytosis

Disease: Epileptic Encephalopathy, Early Infantile, 4
Research Articles on STXBP1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000