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anti-MYOT antibody :: Rabbit anti-Human, Mouse MYOT Polyclonal Antibody

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Catalog # MBS1493502
Unit / Price
  0.05 mL  /  $120 +1 FREE 8GB USB
  0.1 mL  /  $160 +1 FREE 8GB USB
Product Name

MYOT, Polyclonal Antibody

Popular Item
Also Known As

MYOT Antibody

Product Synonym Names
57 kDa cytoskeletal protein; Myofibrillar titin-like Ig domains protein; Titin immunoglobulin domain protein; MYOT; TTID
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
314
OMIM
159000
3D Structure
ModBase 3D Structure for Q9UBF9
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Antigen Affinity Purified
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human Myotilin protein (443-498aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-15235 / sc-15237
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-MYOT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MYOT antibody
Component of a complex of multiple actin cross-linking proteins. Involved in the control of myofibril assembly and stability at the Z lines in muscle cells.
Applications Tested/Suitable for anti-MYOT antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-MYOT antibody
Recommended dilution: WB:1:500-2000
IHC:1:20-1:200

Western Blot (WB) of anti-MYOT antibody
Western blot
All lanes: MYOT antibody at 2ug/ml
Lane 1:293T whole cell lysate
Lane 2:HepG2 whole cell lysate
Lane 3:mouse skeletal muscle tissue
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 56,36 kDa
Observed band size: 55 kDa

anti-MYOT antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-MYOT antibody
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using MBS1493502 at dilution 1:100
anti-MYOT antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for MYOT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
35,147 Da
NCBI Official Full Name
myotilin isoform b
NCBI Official Synonym Full Names
myotilin
NCBI Official Symbol
NCBI Official Synonym Symbols
MFM3; TTID; TTOD; LGMD1; LGMD1A
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NCBI Protein Information
myotilin
UniProt Protein Name
Myotilin
UniProt Synonym Protein Names
57 kDa cytoskeletal protein; Myofibrillar titin-like Ig domains protein; Titin immunoglobulin domain protein
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
MYOTI_HUMAN
NCBI Summary for MYOT
This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.[provided by RefSeq, Oct 2008]
UniProt Comments for MYOT
MYOT: Component of a complex of multiple actin cross-linking proteins. Involved in the control of myofibril assembly and stability at the Z lines in muscle cells. Defects in MYOT are the cause of limb-girdle muscular dystrophy type 1A (LGMD1A). LGMD1A is an autosomal dominant degenerative myopathy with onset within a mean age of 28 years. LGMD1A is characterized by progressive skeletal muscle weakness of the hip and shoulder girdles, later progressing to include distal weakness, as well as a distinctive dysarthric pattern of speech. Affected muscle exhibits disorganization and streaming of the Z-line. Defects in MYOT are the cause of myopathy myofibrillar type 3 (MFM3). A neuromuscular disorder characterized by progressive skeletal muscle weakness greater distally than proximally, tight heel cords, hyporeflexia, cardiomyopathy and peripheral neuropathy in some patients. Affected muscle exhibits disorganization and streaming of the Z-line, presence of large hyaline structures, excessive accumulation of myotilin and other ectopically expressed proteins and prominent congophilic deposits. Defects in MYOT are the cause of spheroid body myopathy (SBM). SBM is an autosomal dominant form of myofibrillar myopathy (MFM), characterized by slowly progressing proximal muscle weakness and dysarthric nasal speech. There is no evidence of cardiomyopathy. Muscle biopsy shows spheroid bodies within the type I muscle fibers. Belongs to the myotilin/palladin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: actin cytoskeleton; sarcolemma; Z disc

Molecular Function: actin binding; alpha-actinin binding; protein binding; structural constituent of muscle

Biological Process: muscle contraction

Disease: Muscular Dystrophy, Limb-girdle, Type 1a; Myopathy, Myofibrillar, 3; Myopathy, Spheroid Body
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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