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anti-NF2 antibody :: Rabbit anti-Human, Mouse NF2 / Merlin Polyclonal Antibody

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Catalog # MBS240545
Unit / Price
  0.05 mg  /  $530 +1 FREE 8GB USB
Product Name

NF2 / Merlin, Polyclonal Antibody

Popular Item
Full Product Name

Rabbit Polyclonal to Human NF2 / Merlin

Product Synonym Names
Anti-NF2 / Merlin Antibody (phospho-Ser518) IHC-plus; NF2; ACN; BANF; Moesin-ezrin-radixin like; Neurofibromin 2 (merlin); Neurofibromin-2; SCH; Merlin; Schwannomerlin; Schwannomin; Human NF2; Merlin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
phenotype 607379
3D Structure
ModBase 3D Structure for P35240
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Specificity
Human NF2 (Merlin) phospho-peptide corresponding to a region of the human protein surrounding S518 and conjugated to Keyhole Limpet Hemocyanin (KLH).
Purity/Purification
Immunoaffinity Purified
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
1 mg/ml (lot specific)
Target Species
Human
Antigen Modification
pSer518
Preparation and Storage
Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.
Other Notes
Small volumes of anti-NF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NF2 antibody
Mutations linked to neurofibromatosis type 2 led to the identification of NF2. NF2 encodes a protein related to the cytoskeletal components moesin, ezrin, and radixin, and was thus named merlin.
Applications Tested/Suitable for anti-NF2 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
Application Notes for anti-NF2 antibody
ELISA, IHC-P (2.5 ug/ml), IP, WB
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Immunohistochemistry (IHC) of anti-NF2 antibody
Anti-NF2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-NF2 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-NF2 antibody
Anti-Human NF2 (Merlin) pS518 Antibody - Western Blot. Affinity purified phospho-specific antibody to NF2 (Merlin) at pS518 was used at a 1:1000 dilution to detect NF2 by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2, 3 and 4 respectively) for 45. After washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amershams substrate system. Other detection methods will yield similar results.
anti-NF2 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for NF2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
24,515 Da
NCBI Official Full Name
merlin isoform 1
NCBI Official Synonym Full Names
neurofibromin 2 (merlin)
NCBI Official Symbol
NCBI Official Synonym Symbols
ACN; SCH; BANF
  [Similar Products]
NCBI Protein Information
merlin; schwannomin; schwannomerlin; neurofibromin-2; moesin-ezrin-radixin like; moesin-ezrin-radixin-like protein; moesin-ezrin-radizin-like protein; neurofibromin 2 (bilateral acoustic neuroma)
UniProt Protein Name
Merlin
UniProt Synonym Protein Names
Moesin-ezrin-radixin-like protein; Neurofibromin-2; Schwannomerlin; Schwannomin
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
MERL_HUMAN
NCBI Summary for NF2
This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]
UniProt Comments for NF2
Merlin: a moesin-ezrin-radizin-like protein. Interacts with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. Expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations are associated with neurofibromatosis type II. Two predominant isoforms and eight minor isoforms are produced by alternatively spliced transcripts.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Tumor suppressor

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: filopodium membrane; cortical actin cytoskeleton; early endosome; adherens junction; cytoskeleton; extrinsic to membrane; membrane; lamellipodium; perinuclear region of cytoplasm; apical part of cell; cytoplasm; plasma membrane; nucleolus; nucleus; cleavage furrow

Molecular Function: protein binding; actin binding

Biological Process: intercellular junction assembly and maintenance; negative regulation of MAPKKK cascade; hippocampus development; regulation of protein stability; negative regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of cell-matrix adhesion; odontogenesis of dentine-containing teeth; Schwann cell proliferation; negative regulation of cell proliferation; mesoderm formation; negative regulation of DNA replication; positive regulation of stress fiber formation; negative regulation of tyrosine phosphorylation of Stat3 protein; ectoderm development; negative regulation of protein kinase activity; negative regulation of cell-cell adhesion; actin cytoskeleton organization and biogenesis; positive regulation of cell differentiation; negative regulation of JAK-STAT cascade; negative regulation of cell migration

Disease: Meningioma, Familial, Susceptibility To; Neurofibromatosis, Type Ii; Schwannomatosis 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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