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anti-NFS1 antibody :: Rabbit anti-Human, Mouse NFS1 Polyclonal Antibody

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Catalog # MBS7045600
Unit / Price
  0.05 mL  /  $120 +1 FREE 8GB USB
  0.1 mL  /  $160 +1 FREE 8GB USB
Product Name

NFS1, Polyclonal Antibody

Popular Item
Full Product Name

NFS1 Antibody

Product Synonym Names
Cysteine desulfurase, mitochondrial; NFS1; NIFS; HUSSY-08
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
603485
3D Structure
ModBase 3D Structure for Q9Y697
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Species
Human
Immunogen
Recombinant human Cysteine desulfurase, mitochondrial protein (C-250AA)
Conjugate
Non-conjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-85785 / sc-85784 / sc-85786 / sc-98420
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-NFS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NFS1 antibody
Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor.
Applications Tested/Suitable for anti-NFS1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Western Blot (WB) of anti-NFS1 antibody
Western blot
All lanes: NFS1 antibody at 6.21ug/ml
Lane 1:HepG2 whole cell lysate
Lane 2:Hela whole cell lysate
Lane 3:mouse kidney tissue
Lane 4:K562 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 51,45 kDa
Observed band size: 50 kDa,85 kDa

anti-NFS1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-NFS1 antibody
Immunohistochemistry of paraffin-embedded human colon cancer tissue using MBS7045600 at dilution 1:100
anti-NFS1 antibody Immunohistochemistry (IHC) (IHC) image
Immunoprecipitation (IP) of anti-NFS1 antibody
Immunoprecipitating NFS1 in HeLa whole cell lysate
Lane 1: Rabbit monoclonal IgG(1ug)instead of MBS7045600 in HeLa whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: MBS7045600(4ug)+ HeLa whole cell lysate(500ug)
Lane 3: HeLa whole cell lysate (20ug)

anti-NFS1 antibody Immunoprecipitation (IP) (IP) image
NCBI/Uniprot data below describe general gene information for NFS1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
44,362 Da
NCBI Official Full Name
cysteine desulfurase, mitochondrial isoform b
NCBI Official Synonym Full Names
NFS1, cysteine desulfurase
NCBI Official Symbol
NCBI Official Synonym Symbols
IscS; NIFS; HUSSY-08
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NCBI Protein Information
cysteine desulfurase, mitochondrial
UniProt Protein Name
Cysteine desulfurase, mitochondrial
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
NFS1_HUMAN
NCBI Summary for NFS1
Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2010]
UniProt Comments for NFS1
NFS1: Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron- sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: EC 2.8.1.7; Transferase; Mitochondrial; Cofactor and Vitamin Metabolism - thiamine

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; mitochondrial matrix; mitochondrion; nucleolus; nucleoplasm; nucleus

Molecular Function: cysteine desulfurase activity; protein binding; protein homodimerization activity

Biological Process: iron incorporation into metallo-sulfur cluster; molybdopterin cofactor biosynthetic process; protein complex assembly; sulfur amino acid metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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