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anti-NT5E antibody :: Rabbit anti-Human, Mouse NT5E / eNT / CD73 Polyclonal Antibody

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Catalog # MBS242461
Unit / Price
  0.2 mL  /  $530 +1 FREE 8GB USB
Product Name

NT5E / eNT / CD73, Polyclonal Antibody

Popular Item
Full Product Name

Rabbit Polyclonal to Human NT5E / eNT / CD73

Product Synonym Names
Anti-NT5E / eNT / CD73 Antibody (C-Terminus) IHC-plus; NT5E; 5-nucleotidase; ecto (CD73); 5'-nucleotidase; 5 nucleotidase (CD73); 5-NT; 5-nucleotidase; 5'-nucleotidase; ecto (CD73); CD73; CD73 antigen; E5NT; Ecto-5-nucleotidase; ENT; EN; NT; Purine 5-Prime-Nucleotidase; NT5; NTE; Human NT5E; eNT; CD73
Product Synonym Gene Name
eNT; CD73[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
gene 211800
3D Structure
ModBase 3D Structure for P21589
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Specificity
Cross-reactivity with mouse has been confirmed in WB only. The antibody has not been evaluated for use in IHC with mouse tissues at time.
Purity/Purification
Ammonium sulfate precipitation
Form/Format
PBS, 0.09% sodium azide
Target Species
Human
Immunogen Description
KLH conjugated synthetic peptide selected from the C-terminal region of human NT5E.
Immunogen Type
Synthetic peptide
Immunogen
NT5E / eNT / CD73 antibody was raised against kLH conjugated synthetic peptide selected from the C-terminal region of human NT5E.
Antigen Modification
C-Terminus
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
Other Notes
Small volumes of anti-NT5E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-NT5E antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-NT5E antibody
IHC-P (10 ug/ml), WB (1:1000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-NT5E antibody
Anti-NT5E / CD73 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
anti-NT5E antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-NT5E antibody
Formalin-fixed and paraffin-embedded human Placenta tissue reacted with CD73 (NT5E) Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
anti-NT5E antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-NT5E antibody
Western blot of mouse brain tissue lysate.
anti-NT5E antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-NT5E antibody
Western blot analysis of CD73 Pab in mouse brain tissue lysate. CD73 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
anti-NT5E antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-NT5E antibody
Western blot analysis of NT5E (arrow) using rabbit polyclonal NT5E Antibody (M535). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NT5E gene.
anti-NT5E antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for NT5E. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,949 Da
NCBI Official Full Name
5'-nucleotidase isoform 1 preproprotein
NCBI Official Synonym Full Names
5'-nucleotidase, ecto (CD73)
NCBI Official Symbol
NCBI Official Synonym Symbols
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
  [Similar Products]
NCBI Protein Information
5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; Purine 5-Prime-Nucleotidase
UniProt Protein Name
5'-nucleotidase
UniProt Synonym Protein Names
Ecto-5'-nucleotidase; CD_antigen: CD73
UniProt Gene Name
UniProt Synonym Gene Names
NT5; NTE; 5'-NT  [Similar Products]
UniProt Entry Name
5NTD_HUMAN
NCBI Summary for NT5E
The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
UniProt Comments for NT5E
NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Phosphatase (non-protein); Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; EC 3.1.3.5; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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