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anti-SPP1 antibody :: Rabbit Osteopontin Polyclonal Antibody

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Catalog # MBS7602266
Unit / Price
  0.1 mg  /  $290 +1 FREE 8GB USB
Product Name

Osteopontin (SPP1), Polyclonal Antibody

Popular Item
Also Known As

Osteopontin Rabbit Polyclonal

Product Synonym Names
BNSP, Bone sialoprotein 1, BSPI, ETA 1, Nephropontin, OPN, Osteopontin, secreted phosphoprotein 1, SPP 1, SPP1, Urinary stone protein, Uropontin
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS7602266 Technical Datasheet
Immunogen Sequence Length
300
OMIM
166490
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Specificity
Please decide the specificity by homology
Purity/Purification
>=95% as determined by SDS-PAGE
Immunogen Affinity Purified
Form/Format
Liquid
Immunogen
Secreted phosphoprotein 1
Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.3
Calculated MW
66kDa
Preparation and Storage
Store at -20°C for 24 months (Avoid repeated freeze / thaw cycles.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-SPP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SPP1 antibody
Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression.
Applications Tested/Suitable for anti-SPP1 antibody
ELISA (EIA), Immunohiostochemistry (IHC), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-SPP1 antibody
Western Blot (WB) 1:500-1:2000
Immunohistochemistry (IHC) 1:50-1:200
Immunofluorescence (IF) 1:20-1:200

Immunohistochemistry (IHC) of anti-SPP1 antibody
Immunohistochemistry of paraffin-embedded human stomach cancer using MBS7602266 (OPN antibody) at dilution of 1:50
anti-SPP1 antibody Immunohistochemistry (IHC) (IHC) image
Immnofluorescent (IF) of anti-SPP1 antibody
mmunofluorescent analysis of HepG2 cells using MBS7602266 (Osteopontin antibody) at dilution of 1:50 and Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L)
anti-SPP1 antibody Immnofluorescent (IF) image
Testing Data (TD) of anti-SPP1 antibody
mouse kidney tissue were subjected to SDS PAGE followed by western blot with MBS7602266 ( OPN Antibody) at dilution of 1:1000
anti-SPP1 antibody Testing Data (TD) image
NCBI/Uniprot data below describe general gene information for SPP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
osteopontin
NCBI Official Synonym Full Names
secreted phosphoprotein 1
NCBI Official Symbol
NCBI Official Synonym Symbols
OPN; BNSP; BSPI; ETA-1
  [Similar Products]
NCBI Protein Information
osteopontin
UniProt Protein Name
Osteopontin
UniProt Synonym Protein Names
Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
BNSP; OPN; SPP-1  [Similar Products]
UniProt Entry Name
OSTP_HUMAN
NCBI Summary for SPP1
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
UniProt Comments for SPP1
osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: cell projection; extracellular region; extracellular space; perinuclear region of cytoplasm

Molecular Function: cytokine activity; extracellular matrix binding; protein binding

Biological Process: biomineral formation; cell adhesion; decidualization; embryo implantation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; inflammatory response; negative regulation of collateral sprouting of intact axon in response to injury; osteoblast differentiation; positive regulation of bone resorption; response to steroid hormone stimulus; response to vitamin D
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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