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anti-PDE4D antibody :: Goat PDE4D Polyclonal Antibody

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Catalog # MBS421503
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

PDE4D, Polyclonal Antibody

Also Known As

Goat anti-PDE4D Antibody

Product Synonym Names
Goat Anti-PDE4D3 Antibody; PDE4D; phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila); HGNC: 8783; DPDE3; HSPDE4D; PDE4DN2; STRK1; cAMP-specific phosphodiesterase 4D; cAMP-specific phosphodiesterase PDE4D6; dunce-like phosphodiesterase E3; phosphodie; PDE4D antibody; phosphodiesterase 4D; cAMP-specific (phosphodiesterase E3 dunce homolog; Drosophila) antibody; HGNC: 8783 antibody; DPDE3 antibody; HSPDE4D antibody; PDE4DN2 antibody; STRK1 antibody; cAMP-specific phosphodiesterase 4D antibody; cAMP-specific phosphodiesterase PDE4D6 antibody; dunce-like phosphodiesterase E3 antibody; phosphodiesterase 4D; cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E3) antibody; DKFZp686M11213 antibody; FLJ97311 antibody; phosphodiesterase 4D antibody
Antibody/Peptide Pairs
PDE4D peptide (MBS428550) is used for blocking the activity of PDE4D antibody (MBS421503)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
585
Immunogen Sequence
QPEACVIDDR SPDT
OMIM
600129
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence QPEACVIDDRSPDT, from the C Terminus of the protein sequence according to NP_001184151.1; NP_001184150.1; NP_006194.2; NP_001098101.1; NP_001184152.1; NP_001159371.1.
Epitope
C Terminus
Note
This antiobody is expected to recognize all reported isoforms.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-PDE4D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-PDE4D antibody
Peptide ELISA (EIA), Western Blot (WB-Trf), ELISA (EIA)
Application Notes for anti-PDE4D antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 70+80+90kDa bands observed in lysates of cell line A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68kDa and 95kDa are observed. These bands are not observed in the untransfected COS1. In transfected HEK293 transiently expressing PDE4D2 multi bands from approx.55kDa to 80kDa are observed. These bands are not observed in the non-transfected HEK293. Calculated MW of 91.1kDa according to PDE4D1 (NP_001098101.1), 76.5kDa according to PDE4D2 (NP_006194.2) and 84.7kDa according to PDE4D3 (NP_001159371.1). Recommended concentration: 0.1-0.3ug/ml.
ELISA: This antibody has been successfully used in ELISA as described in the following paper: Bazhin et al, Mol Immunol.2010 Jan;47(4): 678-84.; PMID: 19939455.

Western Blot (WB) of anti-PDE4D antibody
Staining (1ug/ml) of COS cell lysates (25ug protein): transfected with Human PDE4D1 (1), transfected with Human PDE4D3 (2), untransfected (3). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-PDE4D antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-PDE4D antibody
HEK293 overexpressing Human PDE4D2 (RC212410) and probed (mock transfection in first lane), tested by Origene.
anti-PDE4D antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for PDE4D. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_001184150.1; NP_006194.2; NP_001098101.1; NP_001184152.1; NP_001159371.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
24,429 Da
NCBI Official Full Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform PDE4D1
NCBI Official Synonym Full Names
phosphodiesterase 4D
NCBI Official Symbol
PDE4D  [Similar Products]
NCBI Official Synonym Symbols
DPDE3; PDE43; STRK1; ACRDYS2; HSPDE4D; PDE4DN2
  [Similar Products]
NCBI Protein Information
cAMP-specific 3',5'-cyclic phosphodiesterase 4D
UniProt Protein Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4D
UniProt Synonym Protein Names
DPDE3; PDE43
UniProt Gene Name
PDE4D  [Similar Products]
UniProt Synonym Gene Names
DPDE3  [Similar Products]
UniProt Entry Name
PDE4D_HUMAN
NCBI Summary for PDE4D
This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009]
UniProt Comments for PDE4D
PDE4D: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1). Isoform 5, isoform N3 and isoform 12 bind GNB2L1 via their unique N-terminus. Binds ARRB2. Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle. Inhibited by rolipram. Activated by phosphatidic acid. Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. 12 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.53; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 5q12

Cellular Component: cytosol; voltage-gated calcium channel complex

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; ATPase binding; beta-2 adrenergic receptor binding; cAMP binding; drug binding; enzyme binding; protein binding

Biological Process: cAMP catabolic process; negative regulation of heart contraction; negative regulation of peptidyl-serine phosphorylation; positive regulation of interferon-gamma production; positive regulation of interleukin-2 production; positive regulation of interleukin-5 production; regulation of heart rate; regulation of receptor activity; T cell receptor signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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