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anti-PER3 antibody :: Rabbit anti-Human, Mouse PER3 Polyclonal Antibody

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Catalog # MBS7052237
Unit / Price
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

PER3, Polyclonal Antibody

Popular Item
Also Known As

PER3 Antibody

Product Synonym Names
Period circadian protein homolog 3; hPER3; Cell growth-inhibiting gene 13 protein; Circadian clock protein PERIOD 3; PER3; GIG13
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1191
OMIM
AB047521 Genomic DNA
3D Structure
ModBase 3D Structure for P56645
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Immunogen
Recombinant Human Period circadian protein homolog 3 protein (1-131AA)
Conjugation
Non-conjugated
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-PER3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PER3 antibody
Originally described as a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals, body temperature and feeding-related cues, aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK, NPAS2, ARNTL/BMAL1, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1 and CRY2) plays a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length, in time, of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle, while the ultradian and infradian rhythms have a period shorter and longer than 24 hours, respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases, cancer, metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1, NR1D2, RORA, RORB and RORG, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. Has a redundant role with the other PER proteins PER1 and PER2 and is not essential for the circadian rhythms maintenance. In contrast, plays an important role in sleep-wake timing and sleep homeostasis probably through the transcriptional regulation of sleep homeostasis-related genes, without influencing circadian parameters. Can bind heme.
Applications Tested/Suitable for anti-PER3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-PER3 antibody
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:200

Western Blot (WB) of anti-PER3 antibody
Western Blot
Positive WB detected in: Hela whole cell lysate,Mouse skeletal muscle tissue
All lanes: PER3 antibody at 4ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 132,133 KDa
Observed band size: 132 KDa

anti-PER3 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-PER3 antibody
Immunohistochemistry of paraffin-embedded human ovarian cancer using MBS7052237 at dilution of 1:100
anti-PER3 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-PER3 antibody
Immunohistochemistry of paraffin-embedded human colon cancer using MBS7052237 at dilution of 1:100
anti-PER3 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-PER3 antibody
Immunofluorescent analysis of Hela cells using MBS7052237 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-PER3 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for PER3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
132,660 Da
NCBI Official Full Name
period circadian protein homolog 3 isoform 2
NCBI Official Synonym Full Names
period circadian clock 3
NCBI Official Symbol
NCBI Official Synonym Symbols
GIG13; FASPS3
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NCBI Protein Information
period circadian protein homolog 3
UniProt Protein Name
Period circadian protein homolog 3
UniProt Synonym Protein Names
Cell growth-inhibiting gene 13 protein; Circadian clock protein PERIOD 3
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
hPER3  [Similar Products]
NCBI Summary for PER3
This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been linked to sleep disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2014]
UniProt Comments for PER3
PER3: Component of the circadian clock mechanism which is essential for generating circadian rhythms. Can bind heme. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 1p36.23

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: negative regulation of transcription from RNA polymerase II promoter; protein stabilization; regulation of circadian sleep/wake cycle, sleep

Disease: Advanced Sleep Phase Syndrome, Familial, 3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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