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anti-ABL1 antibody :: Rabbit Phospho-ABL (Y412) Polyclonal Antibody

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Catalog # MBS9204367
Unit / Price
  0.08 mL  /  $150 +1 FREE 8GB USB
  0.4 mL  /  $340 +1 FREE 8GB USB
Product Name

Phospho-ABL (Y412) (ABL1), Polyclonal Antibody

Also Known As

Phospho-ABL (Y412) Antibody

Product Synonym Names
Tyrosine-protein kinase ABL1; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150; ABL1; ABL; JTK7
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1,149
OMIM
189980
3D Structure
ModBase 3D Structure for P00519
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human (Predicted Reactivity: Mouse)
Specificity
This ABL Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y412 of human ABL.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.34 (lot specific)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-ABL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ABL1 antibody
ABL2 is a cytoplasmic tyrosine kinase which is closely related to but distinct from ABL1. The similarity of the proteins includes the tyrosine kinase domains and extends amino-terminal to include the SH2 and SH3 domains. ABL2 is expressed in both normal and tumor cells.
Applications Tested/Suitable for anti-ABL1 antibody
Western Blot (WB), Dot Blot (DB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-ABL1 antibody
WB~~1:1000

Western Blot (WB) of anti-ABL1 antibody
Western blot analysis of Phospho-ABL-Y412 Antibody Pab in A2058 cell line lysate (35ug/lane). ABL (arrow) was detected using the purified Pab.
anti-ABL1 antibody Western Blot (WB) (WB) image
Dot Blot (DB) of anti-ABL1 antibody
Dot blot analysis of ABL-Y412 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
anti-ABL1 antibody Dot Blot (DB) image
Immunohistochemistry (IHC) of anti-ABL1 antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
anti-ABL1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ABL1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Human (Predicted Reactivity: Mouse)NP_009297.2[Other Products]
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
122873
NCBI Official Full Name
tyrosine-protein kinase ABL1 isoform a
NCBI Official Synonym Full Names
ABL proto-oncogene 1, non-receptor tyrosine kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl
  [Similar Products]
NCBI Protein Information
tyrosine-protein kinase ABL1
UniProt Protein Name
Tyrosine-protein kinase ABL1
UniProt Synonym Protein Names
Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
ABL; JTK7  [Similar Products]
UniProt Entry Name
ABL1_HUMAN
NCBI Summary for ABL1
This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [provided by RefSeq, Aug 2014]
UniProt Comments for ABL1
Abl: an ubiquitously expressed and highly conserved proto-oncogenic tyrosine kinase. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. Negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion and stress response. The Philadephia chromosome translocation t(9;22)(q34;q11) creates a Bcr-Abl fusion protein, responsible for 90% of chronic myelogenous leukemia (CML) and ~25% of acute lymphoblastic leukemia (ALL). Inhibitors: Gleevec (imatinib, Glivec), Dasatinib. Two alternatively-spliced isoforms have been described.

Protein type: Protein kinase, TK; Kinase, protein; Oncoprotein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; TK group; Abl family

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: nucleoplasm; extrinsic to internal side of plasma membrane; nuclear membrane; mitochondrion; perinuclear region of cytoplasm; leading edge; cytoplasm; nucleolus; cytosol; nucleus; actin cytoskeleton

Molecular Function: actin monomer binding; protein C-terminus binding; non-membrane spanning protein tyrosine kinase activity; magnesium ion binding; protein kinase activity; actin filament binding; protein binding; DNA binding; syntaxin binding; protein-tyrosine kinase activity; manganese ion binding; mitogen-activated protein kinase binding; nicotinate-nucleotide adenylyltransferase activity; SH3 domain binding; ATP binding; receptor binding

Biological Process: DNA damage induced protein phosphorylation; positive regulation of apoptosis; cerebellum morphogenesis; negative regulation of BMP signaling pathway; B cell receptor signaling pathway; elevation of cytosolic calcium ion concentration; transitional one stage B cell differentiation; regulation of endocytosis; negative regulation of mitotic cell cycle; neuromuscular process controlling balance; cell cycle arrest; positive regulation of oxidoreductase activity; spleen development; mitosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of I-kappaB kinase/NF-kappaB cascade; muscle cell differentiation; positive regulation of peptidyl-tyrosine phosphorylation; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of osteoblast proliferation; negative regulation of ubiquitin-protein ligase activity; autophagy; response to oxidative stress; actin cytoskeleton organization and biogenesis; axon guidance; peptidyl-tyrosine phosphorylation; alpha-beta T cell differentiation; platelet-derived growth factor receptor signaling pathway; mitochondrial depolarization; positive regulation of mitotic cell cycle; Bergmann glial cell differentiation; regulation of transcription, DNA-dependent; collateral sprouting; cell differentiation; B cell proliferation during immune response; epidermal growth factor receptor signaling pathway; regulation of cell adhesion; cell migration; mismatch repair; thymus development; DNA damage response, signal transduction; B-1 B cell homeostasis; activated T cell proliferation; protein modification process; regulation of cell proliferation; microspike biogenesis; positive regulation of muscle cell differentiation; innate immune response; regulation of autophagy; negative regulation of cell-cell adhesion; positive regulation of release of sequestered calcium ion into cytosol; blood coagulation; response to DNA damage stimulus

Disease: Leukemia, Chronic Myeloid
Product References and Citations for anti-ABL1 antibody
Cao, C., et al., Biochemistry 42(35):10348-10353 (2003). Cao, C., et al., J. Biol. Chem. 278(32):29667-29675 (2003). Kruh, G.D., et al., Proc. Natl. Acad. Sci. U.S.A. 87(15):5802-5806 (1990). Kruh, G.D., et al., Science 234(4783):1545-1548 (1986). Bianchi, C., et al., FEBS Lett. 527 (1-3), 216-222 (2002) (): ().

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