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anti-PLOD1 antibody :: Rabbit anti-Human, Mouse PLOD1 Polyclonal Antibody

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Catalog # MBS9128118 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
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  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $275 +1 FREE 8GB USB
  0.2 mL  /  $430 +1 FREE 8GB USB
Western Blot (WB)
Product Name

PLOD1, Polyclonal Antibody

Popular Item
Full Product Name

PLOD1 Polyclonal Antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
153454
3D Structure
ModBase 3D Structure for Q02809
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Affinity Purification
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human PLOD1
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-PLOD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PLOD1 antibody
Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity.
Applications Tested/Suitable for anti-PLOD1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PLOD1 antibody
WB 1:500 - 1:2000
IHC 1:50 - 1:200

Western Blot (WB) of anti-PLOD1 antibody
Western blot analysis of extracts of various cell lines, using PLOD1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
anti-PLOD1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for PLOD1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
84kDa
NCBI Official Full Name
procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
NCBI Official Synonym Full Names
procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1
NCBI Official Symbol
PLOD1  [Similar Products]
NCBI Official Synonym Symbols
LH; LH1; LLH; EDS6; PLOD
  [Similar Products]
NCBI Protein Information
procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
UniProt Protein Name
Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
UniProt Synonym Protein Names
Lysyl hydroxylase 1; LH1
UniProt Gene Name
PLOD1  [Similar Products]
UniProt Synonym Gene Names
LLH; PLOD; LH1  [Similar Products]
UniProt Entry Name
PLOD1_HUMAN
NCBI Summary for PLOD1
Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. [provided by RefSeq, Jul 2008]
UniProt Comments for PLOD1
PLOD1: Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. Defects in PLOD1 are the cause of Ehlers-Danlos syndrome type 6 (EDS6). EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS6 is characterized by the presence of ocular complications, particularly retinal detachment. Defects in PLOD1 are the cause of Nevo syndrome (NEVOS). This is a rare, autosomal recessive disorder characterized by increased perinatal length, kyphosis, muscular hypotonia, and joint laxity. Nevo syndrome and EDS-VI have similar clinical phenotypes. Some authors consider that both syndromes are the same clinical entity.

Protein type: Amino Acid Metabolism - lysine degradation; Oxidoreductase; EC 1.14.11.4

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: endoplasmic reticulum membrane; rough endoplasmic reticulum membrane

Molecular Function: protein homodimerization activity; L-ascorbic acid binding; iron ion binding; procollagen-lysine 5-dioxygenase activity

Biological Process: hydroxylysine biosynthetic process; extracellular matrix organization and biogenesis; epidermis development; response to hypoxia; protein modification process

Disease: Ehlers-danlos Syndrome, Type Vi
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with anti-PLOD1 antibody
 Reference Product  PubMed Publications
 PLOD3 antibody  >7 publications with PLOD1 and PLOD3
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