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anti-PREX2 antibody :: Rabbit anti-Human, Mouse PREX2 Polyclonal Antibody

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Catalog # MBS7050465
Unit / Price
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

PREX2, Polyclonal Antibody

Popular Item
Full Product Name

PREX2 Antibody

Product Synonym Names
Phosphatidylinositol 3; 4; 5-trisphosphate-dependent Rac exchanger 2 proteinP-Rex2PtdIns(3; 4; 5)-dependent Rac exchanger 2; DEP domain-containing protein 2; PREX2; DEPDC2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
AJ437636 mRNA
3D Structure
ModBase 3D Structure for Q70Z35
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Immunogen
Recombinant human Phosphatidylinositol 3, 4, 5-trisphosphate-dependent Rac exchanger 2 protein (1417-1592AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-PREX2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PREX2 antibody
Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3, 4, 5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase.
Applications Tested/Suitable for anti-PREX2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-PREX2 antibody
WB: 1: 2000-1: 5000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200

Western Blot (WB) of anti-PREX2 antibody
Western Blot
Positive WB detected in:Mouse brain tissue
All lanes: PREX2 antibody at 3.4ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 183,172,113 kDa
Observed band size: 183,113 kDa

anti-PREX2 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-PREX2 antibody
Immunohistochemistry of paraffin-embedded human renal tissue using MBS7050465 at dilution of 1:100
anti-PREX2 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-PREX2 antibody
Immunofluorescent analysis of HepG2 cells using MBS7050465 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-PREX2 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for PREX2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
112,404 Da
NCBI Official Full Name
phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein isoform a
NCBI Official Synonym Full Names
phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 2
NCBI Official Symbol
PREX2  [Similar Products]
NCBI Official Synonym Symbols
DEP.2; DEPDC2; P-REX2; PPP1R129
  [Similar Products]
NCBI Protein Information
phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein
UniProt Protein Name
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein
UniProt Synonym Protein Names
DEP domain-containing protein 2
UniProt Gene Name
PREX2  [Similar Products]
UniProt Synonym Gene Names
DEPDC2; P-Rex2; PtdIns(3,4,5)-dependent Rac exchanger 2  [Similar Products]
UniProt Entry Name
PREX2_HUMAN
NCBI Summary for PREX2
The protein encoded by this gene belongs to the phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent Rac exchanger (PREX) family, which are Dbl-type guanine-nucleotide exchange factors for Rac family small G proteins. Structural domains of this protein include the catalytic diffuse B-cell lymphoma homology and pleckstrin homology (DHPH) domain, two disheveled, EGL-10, and pleckstrin homology (DEP) domains, two PDZ domains, and a C-terminal inositol polyphosphate-4 phosphatase (IP4P) domain that is found in one of the isoforms. This protein facilitates the exchange of GDP for GTP on Rac1, allowing the GTP-bound Rac1 to activate downstream effectors. Studies also show that the pleckstrin homology domain of this protein interacts with the phosphatase and tensin homolog (PTEN) gene product to inhibit PTEN phosphatase activity, thus activating the phosphoinositide-3 kinase (PI3K) signaling pathway. Conversely, the PTEN gene product has also been shown to inhibit the GEF activity of this protein. This gene plays a role in insulin-signaling pathways, and either mutations or overexpression of this gene have been observed in some cancers. [provided by RefSeq, Apr 2016]
UniProt Comments for PREX2
PREX2: Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein- coupled receptors and phosphoinositide 3-kinase. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, Rac/Rho; Phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 8q13.2

Cellular Component: cytoplasm; plasma membrane

Molecular Function: GTPase activator activity; Rac guanyl-nucleotide exchange factor activity

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of protein kinase activity; negative regulation of TOR signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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