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anti-RAD51 antibody :: anti-Human, Mouse RAD51A Polyclonal Antibody

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Catalog # MBS8242715
Unit / Price
  0.03 mL  /  $165 +1 FREE 8GB USB
  0.1 mL  /  $245 +1 FREE 8GB USB
  0.2 mL  /  $350 +1 FREE 8GB USB
Product Name

RAD51A, Polyclonal Antibody

Popular Item
Full Product Name

Anti-RAD51A (pY315) Antibody

Product Synonym Names
RAD51A; RECA; DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog A
Antibody/Peptide Pairs
RAD51A peptide (MBS8243598) is used for blocking the activity of RAD51A antibody (MBS8242715)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
114480
3D Structure
ModBase 3D Structure for Q06609
Clonality
Polyclonal
Species Reactivity
Human, Mouse
Specificity
Recognizes endogenous levels of RAD51A (pY315) protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Source
Rabbit
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RAD51A. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-RAD51 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RAD51 antibody
Rabbit polyclonal antibody to RAD51A (pY315)
Applications Tested/Suitable for anti-RAD51 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-RAD51 antibody
Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200)

Western Blot (WB) of anti-RAD51 antibody
Western blot analysis of RAD51A (pY315) expression in HeLa UV-treated (A), HEK293T UV-treated (B) whole cell lysates.
anti-RAD51 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-RAD51 antibody
Immunohistochemical analysis of RAD51A (pY315) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-RAD51 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for RAD51. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
DNA repair protein RAD51 homolog 1
NCBI Official Synonym Full Names
RAD51 recombinase
NCBI Official Symbol
RAD51  [Similar Products]
NCBI Official Synonym Symbols
RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
  [Similar Products]
NCBI Protein Information
DNA repair protein RAD51 homolog 1
UniProt Protein Name
DNA repair protein RAD51 homolog 1
UniProt Synonym Protein Names
RAD51 homolog A
Protein Family
UniProt Gene Name
RAD51  [Similar Products]
UniProt Synonym Gene Names
RAD51A; RECA; HsRAD51; hRAD51  [Similar Products]
UniProt Entry Name
RAD51_HUMAN
NCBI Summary for RAD51
The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
UniProt Comments for RAD51
Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR). Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair (PubMed:26253028).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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