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anti-RARA antibody :: Rabbit anti-Human, mouse RARA Polyclonal Antibody

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Catalog # MBS9212799
Unit / Price
  0.08 mL  /  $165 +1 FREE 8GB USB
  0.4 mL  /  $370 +1 FREE 8GB USB
Product Name

RARA, Polyclonal Antibody

Popular Item
Full Product Name

RARA Antibody (C-term)

Product Synonym Names
Retinoic acid receptor alpha; RAR-alpha; Nuclear receptor subfamily 1 group B member 1; RARA; NR1B1
Antibody/Peptide Pairs
RARA peptide (MBS9220242) is used for blocking the activity of RARA antibody (MBS9212799)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
322-349
OMIM
180240
3D Structure
ModBase 3D Structure for P10276
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human, mouse
Specificity
This RARA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-349 amino acids from the C-terminal region of human RARA.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
0.31mg/ml (lot specific)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Cellular Location
Nucleus. Cytoplasm. Note=Nuclear localization depends on ligand binding, phosphorylation and sumoylation. Transloaction to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-RARA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RARA antibody
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand- dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis.
Applications Tested/Suitable for anti-RARA antibody
Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes for anti-RARA antibody
WB~~1:1000
IF~~1:10~50
IHC-P~~1:10~50
FC~~1:10~50

Western Blot (WB) of anti-RARA antibody
RARA Antibody (C-term) western blot analysis in HL-60 cell line and mouse testis tissue lysates (35ug/lane).This demonstrates the RARA antibody detected the RARA protein (arrow).
anti-RARA antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-RARA antibody
Confocal immunofluorescent analysis of RARA Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
anti-RARA antibody Immunofluorescence (IF) image
Immunohistochemistry (IHC) of anti-RARA antibody
RARA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RARA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-RARA antibody Immunohistochemistry (IHC) (IHC) image
Flow Cytometry (FC/FACS) of anti-RARA antibody
RARA Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-RARA antibody Flow Cytometry (FC/FACS) (FC/FACS) image
NCBI/Uniprot data below describe general gene information for RARA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
50771
NCBI Official Full Name
retinoic acid receptor alpha isoform 1
NCBI Official Synonym Full Names
retinoic acid receptor, alpha
NCBI Official Symbol
NCBI Official Synonym Symbols
RAR; NR1B1
  [Similar Products]
NCBI Protein Information
retinoic acid receptor alpha
UniProt Protein Name
Retinoic acid receptor alpha
UniProt Synonym Protein Names
Nuclear receptor subfamily 1 group B member 1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
NR1B1; RAR-alpha  [Similar Products]
UniProt Entry Name
RARA_HUMAN
NCBI Summary for RARA
This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.[provided by RefSeq, Sep 2010]
UniProt Comments for RARA
RARA: is a receptor for retinoic acid, a potent mammalian morphogen and teratogen that has profound effects on vertebrate development. RARA is a member of the nuclear receptor superfamily. Controls cell function by directly regulating gene expression. Its phosphorylation is crucial for transcriptional activity. Aberrations involving RARA may be a cause of acute promyelocytic leukemia. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Oncoprotein; Transcription factor; Nuclear receptor

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; cell surface; cell soma; perinuclear region of cytoplasm; nuclear chromatin; dendrite; cytoplasm; nucleus; actin cytoskeleton

Molecular Function: protein domain specific binding; protein kinase B binding; retinoic acid binding; zinc ion binding; chromatin DNA binding; transcription coactivator activity; translation repressor activity, nucleic acid binding; drug binding; phosphoinositide 3-kinase regulator activity; alpha-actinin binding; transcription factor binding; protein binding; enzyme binding; protein heterodimerization activity; protein kinase A binding; steroid hormone receptor activity; mRNA 5'-UTR binding; retinoic acid receptor activity; transcription factor activity; transcription corepressor activity; receptor binding

Biological Process: prostate gland development; negative regulation of translational initiation; estrogen receptor signaling pathway; regulation of myelination; glandular epithelial cell development; positive regulation of transcription, DNA-dependent; regulation of synaptic plasticity; female pregnancy; regulation of phosphoinositide 3-kinase activity; protein amino acid phosphorylation; response to vitamin A; germ cell development; Sertoli cell fate commitment; positive regulation of T-helper 2 cell differentiation; positive regulation of cell cycle; positive regulation of phosphoinositide 3-kinase cascade; response to ethanol; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; limb development; retinoic acid receptor signaling pathway; ventricular cardiac muscle cell differentiation; negative regulation of transcription from RNA polymerase II promoter; signal transduction; response to estradiol stimulus; negative regulation of granulocyte differentiation; positive regulation of interleukin-4 production; negative regulation of cell proliferation; ureteric bud development; negative regulation of interferon-gamma production; positive regulation of cell proliferation; positive regulation of interleukin-13 production; transmembrane transport; positive regulation of interleukin-5 production; transcription initiation from RNA polymerase II promoter; response to retinoic acid; multicellular organism growth; negative regulation of tumor necrosis factor production; positive regulation of binding; liver development; embryonic camera-type eye development; positive regulation of protein kinase B signaling cascade; response to cytokine stimulus; neural tube closure; gene expression; spermatogenesis; positive regulation of neuron differentiation; apoptotic cell clearance

Disease: Acute Promyelocytic Leukemia
Product References and Citations for anti-RARA antibody
Park, U.H., et al. J. Biol. Chem. 285(44):34269-34278(2010)
Asleson, A.D., et al. Cancer Genet. Cytogenet. 202(1):33-37(2010)
Catalano, A., et al. Blood 110(12):4073-4076(2007)
Wells, R.A., et al. Nat. Genet. 17(1):109-113(1997)
Chen, Z., et al. Proc. Natl. Acad. Sci. U.S.A. 91(3):1178-1182(1994)

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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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