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anti-RAB5A antibody :: Rabbit anti-Human, Mouse Ras-related protein Rab-5A Polyclonal Antibody

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Catalog # MBS1499177
Unit / Price
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  0.05 mL  /  $120 +1 FREE 8GB USB
  0.1 mL  /  $160 +1 FREE 8GB USB
Product Name

Ras-related protein Rab-5A (RAB5A), Polyclonal Antibody

Popular Item
Full Product Name

Rabbit anti-human Ras-related protein Rab-5A polyclonal Antibody(RAB5A)

Product Synonym Names
RAB5A; RAB5
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
179512
3D Structure
ModBase 3D Structure for P20339
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Purity/Purification
Antigen Affinity Purified
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human Ras-related protein Rab-5A protein(1-215aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-28570 / sc-130010 / sc-309 / sc-26566
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-RAB5A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RAB5A antibody
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension.
Applications Tested/Suitable for anti-RAB5A antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-RAB5A antibody
Recommended dilution: WB:1:500-5000
IHC:1:20-1:200

Western Blot (WB) of anti-RAB5A antibody
All lanes: Ras-related protein Rab-5A antibody at 5ug/ml
Lane 1:mouse brain tissue
Lane 2:HL-60 whole cell lysate
Lane 3:Hela whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 24,23 kDa
Observed band size: 24 kDa

anti-RAB5A antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-RAB5A antibody
Immunohistochemistry of paraffin-embedded human rectal cancer using MBS1499177 at dilution 1:100
anti-RAB5A antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for RAB5A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
22,178 Da
NCBI Official Full Name
ras-related protein Rab-5A isoform 2
NCBI Official Synonym Full Names
RAB5A, member RAS oncogene family
NCBI Official Symbol
RAB5A  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
ras-related protein Rab-5A
UniProt Protein Name
Ras-related protein Rab-5A
Protein Family
UniProt Gene Name
RAB5A  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
RAB5A_HUMAN
UniProt Comments for RAB5A
RAB5A: Required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, RABEP1, SUN2, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3. Interacts with PIK3CB. Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric, Rab; G protein, monomeric; Motility/polarity/chemotaxis; G protein

Chromosomal Location of Human Ortholog: 3p24-p22

Cellular Component: actin cytoskeleton; axon; cell soma; cytoplasm; cytosol; dendrite; early endosome; endocytic vesicle; endosome; endosome membrane; lipid raft; melanosome; nerve terminal; phagocytic vesicle; plasma membrane; ruffle; synaptic vesicle; terminal button

Molecular Function: GDP binding; GTP binding; GTPase activity; protein binding

Biological Process: blood coagulation; early endosome to late endosome transport; endocytosis; positive regulation of exocytosis; protein transport; regulation of endocytosis; regulation of endosome size; regulation of filopodium formation; small GTPase mediated signal transduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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