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anti-SPTLC1 antibody :: Rabbit anti-Human, Mouse Serine Palmitoyltransferase Polyclonal Antibody

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Catalog # MBS2032142 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $185 +1 FREE 8GB USB
  0.1 mg  /  $230 +1 FREE 8GB USB
  0.2 mg  /  $345 +1 FREE 8GB USB
  1 mg  /  $825 +1 FREE 8GB USB
Product Name

Serine Palmitoyltransferase (SPTLC1), Polyclonal Antibody

Popular Item
Also Known As

Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) Polyclonal Antibody

Product Synonym Names
HSAN; HSAN1; HSN1; LBC1; LCB1; SPT1; SPTI; Hereditary Sensory Neuropathy, Type 1; Long chain base biosynthesis protein 1; Serine-palmitoyl-CoA transferase 1
Matching Pairs
Unconjugated Antibody: Serine Palmitoyltransferase (MBS2032142)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
Matching Pairs
Unconjugated Antibody: Serine Palmitoyltransferase (MBS2032142)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
482
3D Structure
ModBase 3D Structure for O35704
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Specificity
The antibody is a rabbit polyclonal antibody raised against SPTLC1. It has been selected for its ability to recognize SPTLC1 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
200ug/ml (lot specific)
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Organism Species
Mouse
Fragment
SPTLC1 (Asp143~Leu473)
Quality Control
Content: The quality control contains recombinant SPTLC1 (Asp143~Leu473) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2079301
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of anti-SPTLC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-SPTLC1 antibody
Applications Tested/Suitable for anti-SPTLC1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-SPTLC1 antibody
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-SPTLC1 antibody
Western Blot: Sample: Lane1: Mouse Liver Tissue; Lane2: Mouse Lung Tissue; Lane3: Mouse Brain Tissue; Lane4: Human Jurkat Cells.
anti-SPTLC1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-SPTLC1 antibody
Western Blot: Sample: Recombinant SPTLC1, Mouse.
anti-SPTLC1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-SPTLC1 antibody
DAB staining on IHC-P; Samples: Mouse Liver Tissue.
anti-SPTLC1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-SPTLC1 antibody
DAB staining on IHC-P; Samples: Mouse Uterus Tissue
anti-SPTLC1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-SPTLC1 antibody
DAB staining on IHC-P; Samples: Mouse Kidney Tissue.
anti-SPTLC1 antibody Immunohistochemistry (IHC) (IHC) image
Knockout Validation of anti-SPTLC1 antibody
Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: SPTLC1 knockout Jurkat cell lysate; Predicted MW: 53kd Observed MW: 55kd Primary Ab: 1ug/ml Rabbit Anti-Mouse SPTLC1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)
anti-SPTLC1 antibody Knockout Validation image
NCBI/Uniprot data below describe general gene information for SPTLC1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
52,535 Da
NCBI Official Full Name
serine palmitoyltransferase 1
NCBI Official Synonym Full Names
serine palmitoyltransferase, long chain base subunit 1
NCBI Official Symbol
Sptlc1  [Similar Products]
NCBI Official Synonym Symbols
Lcb1; C77762; AW552086; E030036H05
  [Similar Products]
NCBI Protein Information
serine palmitoyltransferase 1
UniProt Protein Name
Serine palmitoyltransferase 1
UniProt Synonym Protein Names
Long chain base biosynthesis protein 1; LCB 1; Serine-palmitoyl-CoA transferase 1; SPT 1; SPT1
UniProt Gene Name
Sptlc1  [Similar Products]
UniProt Synonym Gene Names
Lcb1; LCB 1; SPT 1; SPT1  [Similar Products]
UniProt Comments for SPTLC1
SPTLC1: Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1- SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Defects in SPTLC1 are the cause of hereditary sensory and autonomic neuropathy type 1A (HSAN1A). The hereditary sensory and autonomic neuropathies are a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN1A is an autosomal dominant axonal neuropathy with onset in the second or third decades. Initial symptoms are loss of pain, touch, heat, and cold sensation over the feet, followed by distal muscle wasting and weakness. Loss of pain sensation leads to chronic skin ulcers and distal amputations. Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.50; Lipid Metabolism - sphingolipid; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 13|13 B1

Cellular Component: endoplasmic reticulum; integral component of membrane; membrane; serine C-palmitoyltransferase complex

Molecular Function: catalytic activity; protein binding; pyridoxal phosphate binding; serine C-palmitoyltransferase activity; transferase activity; transferase activity, transferring acyl groups

Biological Process: biosynthetic process; ceramide biosynthetic process; lipid metabolic process; positive regulation of lipophagy; sphinganine biosynthetic process; sphingolipid biosynthetic process; sphingolipid metabolic process; sphingomyelin biosynthetic process; sphingosine biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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