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anti-PTPN5 antibody :: Rabbit anti-Human, mouse STEP / PTPN5 Polyclonal Antibody

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Catalog # MBS9204706
Unit / Price
  0.08 mL  /  $165 +1 FREE 8GB USB
  0.4 mL  /  $370 +1 FREE 8GB USB
Product Name

STEP / PTPN5, Polyclonal Antibody

Popular Item
Full Product Name

STEP / PTPN5 Antibody (N-Term)

Product Synonym Names
Tyrosine-protein phosphatase non-receptor type 5; Neural-specific protein-tyrosine phosphatase; Striatum-enriched protein-tyrosine phosphatase; STEP; PTPN5
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
168-198
OMIM
176879
3D Structure
ModBase 3D Structure for P54829
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human, mouse
Specificity
This STEP / PTPN5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-198 amino acids from the N-terminal region of human STEP / PTPN5.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (lot specific)
Function
May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.
Cellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-PTPN5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PTPN5 antibody
STEP (striatum-enriched phosphatase) is a neural-specific protein-tyrosine phosphatase, first isolated from the rat brain. The 537-amino acid predicted human protein as isolated from cDNA sequences is between 85 and 90% identical to the mouse and rat sequences. In rat neuronal cell cultures, glutamate-mediated activation of N-methyl-D-aspartate (NMDA) receptors leads to the rapid but transient phosphorylation of extracellular signal-related kinase-2 (ERK2). NMDA-mediated influx of calcium, activates the calcium-dependent phosphatase calcineurin and the resulting dephosphorylation and activation of STEP. STEP then inactivatea ERK2 through tyrosine dephosphorylation and blocks translocation of the kinase to the nucleus. STEP plays a significant role in regulating the ERK activation and downstream signaling in neurons.
Applications Tested/Suitable for anti-PTPN5 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-PTPN5 antibody
WB~~1:1000
IHC-P~~1:10~50

Western Blot (WB) of anti-PTPN5 antibody
Western blot analysis of hSTEP-Q155 in mouse brain tissue lysates (35ug/lane). STEP (arrow) was detected using the purified Pab
anti-PTPN5 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-PTPN5 antibody
Western blot analysis of hSTEP-Q155 in HepG2 cell line lysates (35ug/lane). STEP (arrow) was detected using the purified Pab.
anti-PTPN5 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-PTPN5 antibody
Formalin-fixed and paraffin-embedded human brain tissue reacted with STEP antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
anti-PTPN5 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for PTPN5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
63538
NCBI Official Full Name
tyrosine-protein phosphatase non-receptor type 5 isoform b
NCBI Official Synonym Full Names
protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched)
NCBI Official Symbol
PTPN5  [Similar Products]
NCBI Official Synonym Symbols
STEP; PTPSTEP
  [Similar Products]
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type 5
UniProt Protein Name
Tyrosine-protein phosphatase non-receptor type 5
UniProt Synonym Protein Names
Neural-specific protein-tyrosine phosphatase; Striatum-enriched protein-tyrosine phosphatase; STEP
UniProt Gene Name
PTPN5  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
PTN5_HUMAN
UniProt Comments for PTPN5
STEP: a non-receptor protein-tyrosine phosphatase. STEP (striatum enriched phosphatase) Contains a single phosphatase catalytic domain located at the C-terminus. Two alternatively-spliced messages have been described.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: phosphotyrosine binding; protein tyrosine phosphatase activity

Biological Process: protein amino acid dephosphorylation
Product References and Citations for anti-PTPN5 antibody
Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
Li, X., et al., Genomics 28(3):442-449 (1995).

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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