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anti-XBP1 antibody :: Rabbit anti-Human, Mouse XBP1 Polyclonal Antibody

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Catalog # MBS240773
Unit / Price
  0.05 mg  /  $530 +1 FREE 8GB USB
Product Name

XBP1, Polyclonal Antibody

Popular Item
Also Known As

Rabbit Polyclonal to Human XBP1

Product Synonym Names
Anti-XBP1 Antibody (N-Terminus) IHC-plus; XBP1; X-box binding protein 1; X-box-binding protein 1; XBP2; TREB5; XBP-1; Human XBP1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
261
OMIM
gene 612371
3D Structure
ModBase 3D Structure for P17861
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Specificity
18 amino acid peptide from near the amino terminus of human XBP-1
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.02% sodium azide.
Concentration
1 mg/ml (lot specific)
Target Species
Human
Immunogen Description
Synthetic peptide from human XBP1.
Immunogen Type
Synthetic peptide
Immunogen
XBP1 antibody was raised against synthetic peptide from human XBP1.
Antigen Modification
N-Terminus
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Other Notes
Small volumes of anti-XBP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-XBP1 antibody
XBP1 is a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. It is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator.
Applications Tested/Suitable for anti-XBP1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-XBP1 antibody
IHC-P (20 ug/ml), WB
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Immunohistochemistry (IHC) of anti-XBP1 antibody
Anti-XBP1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
anti-XBP1 antibody Immunohistochemistry (IHC) (IHC) image
Immunocytochemistry (ICC) of anti-XBP1 antibody
Immunocytochemistry of XBP-1 in HepG2 cells with XBP-1 antibody at 10 ug/ml.
anti-XBP1 antibody Immunocytochemistry (ICC) (ICC) image
Western Blot (WB) of anti-XBP1 antibody
Western blot of XBP-1 in HepG2 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
anti-XBP1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for XBP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,148 Da
NCBI Official Full Name
X-box-binding protein 1 isoform XBP1(U)
NCBI Official Synonym Full Names
X-box binding protein 1
NCBI Official Symbol
NCBI Official Synonym Symbols
XBP2; TREB5; XBP-1
  [Similar Products]
NCBI Protein Information
X-box-binding protein 1; tax-responsive element-binding protein 5
UniProt Protein Name
X-box-binding protein 1
UniProt Synonym Protein Names
Tax-responsive element-binding protein 5
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
TREB5; XBP2; XBP-1  [Similar Products]
UniProt Entry Name
XBP1_HUMAN
NCBI Summary for XBP1
This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq, Jul 2008]
UniProt Comments for XBP1
XBP1: a transcription factor essential for hepatocyte growth, the differentiation of plasma cells, immunoglobulin secretion, and the unfolded protein response (UPR). XBP1 mRNA is spliced by IRE1 during the UPR to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. Only the spliced form of XBP1 can activate the UPR efficiently. Activates UPR target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I. Up-regulated by ATF6 via direct binding to the ERSE in response to endoplasmic reticulum stress. Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7). Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature. Two human isoforms are produced by alternative splicing. Isoform 1 is also known as XBP-1U. Isoform 2, also known as XBP-1S, is produced by IRE1 in response to endoplasmic reticulum stress. IRE1 cleaves a 26-bp fragment causing a frameshift of the mRNA transcript.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 22q12.1|22q12

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; endoplasmic reticulum; cytoplasm; integral to membrane; integral to endoplasmic reticulum membrane; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; protease binding; DNA binding; chromatin DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; estrogen receptor binding; protein kinase binding; transcription factor activity

Biological Process: ubiquitin-dependent protein catabolic process; transcription from RNA polymerase II promoter; phosphoinositide 3-kinase cascade; muscle development; apoptosis; exocrine pancreas development; positive regulation of transcription of target genes involved in unfolded protein response; regulation of protein stability; negative regulation of transcription from RNA polymerase II promoter; cellular response to glucose starvation; protein transport; serotonin secretion, neurotransmission; positive regulation of MHC class II biosynthetic process; positive regulation of transcription factor import into nucleus; angiogenesis; cell growth; response to electrical stimulus; fatty acid biosynthetic process; positive regulation of TOR signaling pathway; positive regulation of autophagy; response to drug; positive regulation of histone methylation; protein destabilization; unfolded protein response; organelle organization and biogenesis; cellular response to nutrient; positive regulation of immunoglobulin secretion; liver development; cholesterol homeostasis; positive regulation of immunoglobulin production; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; cellular response to insulin stimulus; positive regulation of T cell differentiation; endothelial cell proliferation; fatty acid homeostasis; neuron development; positive regulation of B cell differentiation; autophagy; positive regulation of fat cell differentiation; immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; sterol homeostasis; vascular endothelial growth factor receptor signaling pathway; negative regulation of apoptosis

Disease: Major Affective Disorder 7
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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