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anti-FTCD antibody :: Goat 58KGolgi protein Polyclonal Antibody

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Catalog # MBS420986
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

58KGolgi protein (FTCD), Polyclonal Antibody

Also Known As

Goat anti-58KGolgi protein(Internal)/FTCD Antibody

Product Synonym Names
Goat Anti-58K Golgi protein(N-Term)/FTCD, Biotinylated Antibody; FTCD; LCHC1; formiminotransferase cyclodeaminase; formimidoyltransferase cyclodeaminase; LC1 autoantigen; FTCD antibody; LCHC1 antibody; formiminotransferase cyclodeaminase antibody; formimidoyltransferase cyclodeaminase antibody; LC1 autoantigen antibody; 58KGolgi protein; 58KGolgi protein(Internal)/FTCD
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
541
Immunogen Sequence
CLREQGRGKD QPGRL
OMIM
229100
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Pig
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence CLREQGRGKDQPGRL, from the internal region of the protein sequence according to NP_006648.1; NP_996848.1.
Epitope
Internal region
Note
Variants (NP_006648.1; NP_996848.1) encode the same protein.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-FTCD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-FTCD antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-FTCD antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Immunohistochemistry: In paraffin embedded Human Liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes. Recommended concentration, 3-5ug/ml.
Western Blot: Approx 60kDa band observed in Human Liver lysates (calculated MW of 58.9kDa according to NP_006648.1and NP_996848.1). Recommended concentration: 0.01-0.03ug/ml.

Western Blot (WB) of anti-FTCD antibody
Staining (0. 01ug/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-FTCD antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-FTCD antibody
(3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-FTCD antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for FTCD. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_996848.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
16,503 Da
NCBI Official Full Name
formimidoyltransferase-cyclodeaminase isoform A
NCBI Official Synonym Full Names
formimidoyltransferase cyclodeaminase
NCBI Official Symbol
NCBI Official Synonym Symbols
LCHC1
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NCBI Protein Information
formimidoyltransferase-cyclodeaminase
UniProt Protein Name
Formimidoyltransferase-cyclodeaminase
UniProt Synonym Protein Names
Formiminotransferase-cyclodeaminase; FTCD; LCHC1Including the following 2 domains:Glutamate formimidoyltransferase (EC:2.1.2.5)Alternative name(s):Glutamate formiminotransferase; Glutamate formyltransferase
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
FTCD_HUMAN
NCBI Summary for FTCD
The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Dec 2009]
UniProt Comments for FTCD
FTCD: Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA); also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - histidine; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 2.1.2.5; EC 4.3.1.4; Lyase; Methyltransferase

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; ER-Golgi intermediate compartment; Golgi apparatus; Golgi membrane; smooth endoplasmic reticulum membrane

Molecular Function: formimidoyltetrahydrofolate cyclodeaminase activity; formimidoyltransferase activity; microtubule binding; protein binding

Biological Process: folic acid and derivative metabolic process; histidine catabolic process

Disease: Formiminotransferase Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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